光：科学与应用（英文版）2025，Vol.14Issue(10)：3069-3081,13.DOI:10.1038/s41377-025-01941-8
Time-deterministic cryo-optical microscopy
Time-deterministic cryo-optical microscopy
Kosuke Tsuji 1Momoko Shiozaki 2Xiaowei Zhao 2Heqi Xi 3Kazunori Sugiura 4Shun-ichi Fukushima 4Takumi Kunimoto 3Yoshino Tanabe 3Kentaro Nishida 3Kentaro Mochizuki 5Yoshinori Harada 5Masahito Yamanaka 3Nicholas I.Smith 6Rainer Heintzmann 7Zhiheng Yu 2Meng C.Wang 2Takeharu Nagai 8Hideo Tanaka 5Katsumasa Fujita 9Yasuaki Kumamoto 10Shoko Tamura 5Wakana Miyamura 3Toshiki Kubo 11Kenta Mizushima 1Kakeru Kono 3Hanae Hirano3
作者信息
- 1. Department of Applied Physics,Graduate School of Engineering,The University of Osaka,Osaka,Japan||Advanced Photonics and Biosensing Open Innovation Laboratory,AIST-Osaka University,Osaka,Japan
- 2. Janelia Research Campus,Howard Hughes Medical Institute,Ashburn,VA,USA
- 3. Department of Applied Physics,Graduate School of Engineering,The University of Osaka,Osaka,Japan
- 4. Department of Biomolecular Science and Engineering,SANKEN,The University of Osaka,Osaka,Japan
- 5. Department of Pathology and Cell Regulation,Graduate School of Medical Science,Kyoto Prefectural University of Medicine,Kyoto,Japan
- 6. Immunology Frontier Research Center,The University of Osaka,Osaka,Japan
- 7. Institute of Physical Chemistry and Abbe Center of Photonics,Friedrich-Schiller-University Jena,Jena,Germany||Leibniz Institute of Photonic Technology,Jena,Germany
- 8. Transdimensional Life Imaging Division,Institute for Open and Transdisciplinary Research Initiatives,The University of Osaka,Osaka,Japan||Department of Biomolecular Science and Engineering,SANKEN,The University of Osaka,Osaka,Japan
- 9. Department of Applied Physics,Graduate School of Engineering,The University of Osaka,Osaka,Japan||Advanced Photonics and Biosensing Open Innovation Laboratory,AIST-Osaka University,Osaka,Japan||Transdimensional Life Imaging Division,Institute for Open and Transdisciplinary Research Initiatives,The University of Osaka,Osaka,Japan
- 10. Department of Applied Physics,Graduate School of Engineering,The University of Osaka,Osaka,Japan||Transdimensional Life Imaging Division,Institute for Open and Transdisciplinary Research Initiatives,The University of Osaka,Osaka,Japan
- 11. Department of Applied Physics,Graduate School of Engineering,The University of Osaka,Osaka,Japan||Department of Dermatology,Course of Integrated Medicine,Graduate School of Medicine,The University of Osaka,Osaka,Japan
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Kosuke Tsuji,Momoko Shiozaki,Xiaowei Zhao,Heqi Xi,Kazunori Sugiura,Shun-ichi Fukushima,Takumi Kunimoto,Yoshino Tanabe,Kentaro Nishida,Kentaro Mochizuki,Yoshinori Harada,Masahito Yamanaka,Nicholas I.Smith,Rainer Heintzmann,Zhiheng Yu,Meng C.Wang,Takeharu Nagai,Hideo Tanaka,Katsumasa Fujita,Yasuaki Kumamoto,Shoko Tamura,Wakana Miyamura,Toshiki Kubo,Kenta Mizushima,Kakeru Kono,Hanae Hirano..Time-deterministic cryo-optical microscopy[J].光：科学与应用（英文版）,2025,14(10):3069-3081,13.基金项目
This work was supported by JST-CREST and JST COI-NEXT program under Grant number JPMJCR1925 and JPMJPF2009.This work was also partially supported by JST SPRING under Grant number JPMJSP2138,Deutsche Forschungsgemeinschaft SFB1278(TP C04),and the Leibniz Association(Leibniz Science Campus,InfectoOptics,HotAim 2.0).M.C.W.was supported by Janelia Research Campus,Howard Hughes Medical Institute.The authors thank M.Hayakawa for the isolation of cardiomyocytes from neonatal rats,S.Kawano for the development of SIM software,and S.Sakakihara for the preparation of SIM masks.The authors also acknowledge the Visiting Scientist Program at Janelia Research Campus and the Cryo-EM facility at Janelia Research Campus for the sample preparation and cryo-TEM data collection. （TP C04）
