海南医科大学学报2025，Vol.31Issue(22)：1681-1687,7.DOI:10.13210/j.cnki.jhmu.20241217.003
ERK1/2信号通路在新生小鼠持续性肺动脉高压中发病的作用研究
The role of ERK1/2 signaling pathway in the pathogenesis of persistent pulmonary hypertension in neonatal mice
摘要
Abstract
Objective:To study the role of ERK1/2 signaling pathway in the pathogenesis of persistent pulmonary hyperten-sion PPHN in neonatal mice.Methods:A total of 80 neonatal mice were placed in a hypoxic box and randomly divided into the control group,PPHN group,DMSO group and inhibitor group.Right ventricular systolic pressure(RVSP)was measured by right ventricular puncture through the intercostal space,and the morphology of pulmonary blood vessels was observed by H&E staining,and pulmonary vascular remodeling indexes,such as the ratio of pulmonary vascular wall area to the total area of the tube(WA%)and the ratio of pulmonary arterial wall thickness to the outer diameter of the blood vessel(WT%)were calculated.Ex-pression level of proliferating cell nuclear antigen(PCNA)protein was detected by immunohistochemistry.The expression level of PCNA,ERK1/2 and p-ERK1/2 proteins in lung tissue was detected by Western blot.Results:The PPHN group exhibited ele-vated RVSP,increased right ventricular hypertrophy,increased alveolar destruction,significant thickening of pulmonary artery smooth muscle,and significant increased PCNA and p-ERK1/2 expression compared to the control group.In contrast,PPHN mice with the addition of the ERK1/2 inhibitor U0126 resulted in a reduction in PVSP,an improvement in right ventricular hyper-trophy,a reduction in alveolar destruction,and a significant decrease in pulmonary artery smooth muscle thickening.These effects were accompanied by a notable reduction of PCNA and p-ERK1/2 expression.Conclusion:The ERK1/2 signaling pathway is in-volved in the development of persistent pulmonary hypertension in neonatal mice.关键词
新生儿持续性肺动脉高压(PPHN)/ERK1/2信号通路/肺动脉平滑肌/小鼠Key words
Persistent pulmonary hypertension of the newborn(PPHN)/ERK1/2 signaling pathway/Pulmonary artery smooth muscle/Mice分类
医药卫生引用本文复制引用
刘畅,朱亚平,谷强..ERK1/2信号通路在新生小鼠持续性肺动脉高压中发病的作用研究[J].海南医科大学学报,2025,31(22):1681-1687,7.基金项目
This study was supported by the National Natural Science Foundation of China(82360315) （82360315）
2023 Talent Development Fund of Bingcai Xing-Key Laboratory of the Xinjiang Production and Construction Corps-Clinical Medical Research Center for Children's Diseases of the First Affiliated Hospital of the Xinjiang Production and Construction Corps(CZ001209) 国家自然科学基金(82360315) （CZ001209）
兵财行 2023人才发展基金-兵团重点实验室-第一附属医院兵团儿童疾病临床医学研究中心(CZ001209) （CZ001209）
