萆薢分清饮对急性痛风性关节炎大鼠NF-κB/NLRP3信号通路的影响
Effect of Bixie Fenqing Decoction on NF-κB/NLRP3 signaling pathway in rats with acute gouty arthritis
摘要
Abstract
Objective:To explore the therapeutic impact and mechanism of Bixie Fenqing Decoction on acute gouty arthritis(AGA)model rats.Methods:Male SD rats were randomly assigned to the blank group,model group,Bixie Fenqing Decoction group(23.33 g/kg)and colchicine group(0.3 mg/kg),10 rats per group.The rats received preventive treatment daily for a period of 7 days.On the 5th day of gavage,the AGA model was induced by injecting monosodium urate suspension into ankle except the blank group.The swelling degree,gait and inflammatory index score of ankle joint were measured after modeling.The histopatho-logical changes of ankle synovium were examined using H&E staining,the levels of serum inflammatory factors and ROS were de-tected by ELISA.Serum MDA and GSH levels,SOD and CAT activities were detected by biochemical method.Real-time fluo-rescence quantitative PCR was used to detect NLRP3 and ASC mRNA expression levels within the ankle synovial tissue.The ex-pression of related proteins in the NF-κB/NLRP3 pathway was detected by Western blot.Results:In contrast to the blank group,ankle joint of rats in the model group exhibited markedly increased swelling(P<0.01),demonstrated notably higher scores in both gait assessment and inflammatory indices(P<0.01).After H&E staining,synovial tissue hyperplasia was prominently ob-served,a large amount of inflammatory cell infiltrated,and histopathological scoring indicated an increase(P<0.01).The levels of IL-6,IL-1β,TNF-α,ROS,and MDA in serum were increased(P<0.05),SOD,GSH,and CAT were decreased(P<0.01).In the synovial tissue,there was a upregulation of NLRP3 and ASC mRNA,and increased expression of p-NF-κB p65/NF-κB p65,NLRP3,ASC and cleaved Caspase-1 protein(P<0.01).Compared to the model group,ankle swelling,gait score and inflammatory index score in the Bixie Fenqing Decoction group were significantly decreased(P<0.05),synovial tissue hyperpla-sia and inflammatory cell infiltration were decreased,and histopathologic score were decreased(P<0.01).Serum IL-1β,IL-6,TNF-α,ROS and MDA were significantly decreased(P<0.05),SOD and GSH levels were increased(P<0.01).mRNA ex-pressions of NLRP3 and ASC and protein expression levels of p-NF-κB p65/NF-κB p65,NLRP3,ASC,and cleaved Caspase-1 were decreased in synovial tissues(P<0.05).Conclusion:The Bixie Fenqing Decoction may exert its anti-inflammatory effects in AGA rats through inhibiting the NF-κB/NLRP3 signaling pathway.关键词
萆薢分清饮/急性痛风性关节炎/核因子-κB/NLRP3炎症小体Key words
Bixie Fenqing Decoction/Acute gouty arthritis/NF-κB/NLRP3 inflammasome分类
医药卫生引用本文复制引用
张靖,韩真真,王思宇,苏培培,邵灿灿,李晓冰,何小鹃,郭洪涛..萆薢分清饮对急性痛风性关节炎大鼠NF-κB/NLRP3信号通路的影响[J].海南医科大学学报,2025,31(22):1696-1704,9.基金项目
This study was supported by the General Project of National Natural Science Foundation of China(82274342) （82274342）
Henan Province Traditional Chinese Medicine Inheritance and Innovation Talent Project(Zhongjing Project)Top Notch Talents Project in Traditional Chinese Medicine Discipline(CZ0325-13) （Zhongjing Project）
Special Project for Scientific Research of Traditional Chinese Medicine in Henan Province(2023ZXZX1145) （2023ZXZX1145）
Research Special Project of National Traditional Chinese Medicine Clinical Research Base of Henan Provincial Health Commission(2022JDZX091) （2022JDZX091）
Henan Provincial Science and Technology Research Project(252102311259)国家自然科学基金(82274342) （252102311259）
河南省中医药传承与创新人才工程(仲景工程)中医药学科拔尖人才项目(CZ0325-13) （仲景工程）
河南省中医药科学研究专项项目(2023ZXZX1145) （2023ZXZX1145）
河南省卫生健康委国家中医临床研究基地科研专项(2022JDZX091) （2022JDZX091）
河南省科技攻关项目(252102311259) （252102311259）
