海南医科大学学报2025，Vol.31Issue(22)：1738-1744,7.DOI:10.13210/j.cnki.jhmu.20250117.002
基于网络药理学和实验验证探讨藤茶-青钱柳对预防急性酒精中毒的作用机制
Mechanism of Ampelopsis grossedentata and Cyclocarya paliurus in preventing acute alcoholism based on network pharmacology
摘要
Abstract
Objective:To determine the potential mechanisms of action for Ampelopsis grossedentata and Cyclocarya paliurus in preventing acute alcohol poisoning(AAI)based on network pharmacology.Methods:Network pharmacology was utilized to predict potential targets and signaling pathways associated with the treatment of AAI using Ampelopsis grossedentata and Cyclo-carya paliurus.An AAI mouse model was established to validate the efficacy of Ampelopsis grossedentata and Cyclocarya paliurus in alleviating intoxication and protecting the liver in mice with AAI.Results:A total of 6 active components of Ampelopsis grossed-entata and Cyclocarya paliurus were identified,with core targets including AKT1,IL-6,EGFR,BCL-2,and CASP3,etc.The signaling pathways related to the treatment of AAI by Ampelopsis grossedentata and Cyclocarya paliurus were mainly concentrat-ed in cancer-related pathways,the PI3K/AKT signaling pathway,as well as pathways involved in toxoplasmosis.Animal experi-ments showed that compared to the model group,the latency period of intoxication was significantly prolonged and sleep time was significantly reduced in each treatment group(P<0.001).Compared to the control group,ALT,AST,and TG levels were sig-nificantly increased in the model group,along with elevated ADH and MDA levels in liver tissue,while the content of GSH and SOD was significantly decreased(P<0.001).Compared to the model group,serum ALT,AST,and TG levels were significant-ly decreased in the groups treated with Ampelopsis grossedentata and Cyclocarya paliurus,with significant increases in ADH,GSH,and SOD levels and a significant decrease in MDA content in liver tissue(P<0.001).Conclusion:Combined use of Am-pelopsis grossedentata and Cyclocarya paliurus exhibits excellent effects in preventing intoxication,accelerating alcohol metabo-lism,and protecting the liver,potentially through the activation of the PI3K/AKT signaling pathway.关键词
藤茶/青钱柳/急性酒精中毒/解酒/护肝Key words
Ampelopsis grossedentata/Cyclocarya paliurus/Acute alcohol poisoning/Alleviating hangover/Liver protection分类
医药卫生引用本文复制引用
王晓瑞,李莹,王莹,刘琳,俞琦,赵宾宾,孔明望..基于网络药理学和实验验证探讨藤茶-青钱柳对预防急性酒精中毒的作用机制[J].海南医科大学学报,2025,31(22):1738-1744,7.基金项目
This study was supported by the National Key Research and Development Plan Special Project(2019YFC1708502) 国家重点研发计划"中医药现代化研究"重点专项(2019YFC1708502) （2019YFC1708502）
