华西口腔医学杂志2025，Vol.43Issue(6)：819-828,10.DOI:10.7518/hxkq.2025.2025044
聚己内酯—聚乙二醇—血小板浓缩生长因子复合支架的制备及其对人牙周膜干细胞生物学特性的影响
Preparation of polycaprolactone-polyethylene glycol-concentrated growth factor composite scaffolds and the ef-fects on the biological properties of human periodontal ligament stem cells
摘要
Abstract
Objective This study investigated the effects of a polycaprolactone(PCL)-polyethylene glycol(PEG)scaffold incorporated with concentrated growth factor(CGF)on the adhesion,proliferation,and osteogenic dif-ferentiation of human periodontal ligament stem cells(hPDLSCs).Methods The PCL-PEG-CGF composite scaffold was fabricated using an immersion and freeze-drying technique.Its microstructure,mechanical proper-ties,and biocompatibility were systematically characterized.The hPDLSCs were isolated through enzymatic digestion,and the hPDLSCs were identified through flow cytometry.Third-passage hPDLSCs were seeded onto the composite scaf-folds,and their adhesion,proliferation and osteogenic differentiation were assessed using CCK-8 assays,4',6-diamidino-2-phenylindole(DAPI)staining,alkaline phosphatase(ALP)staining,alizarin red staining,and Western blot analysis of osteogenesis-related proteins[Runt-related transcription factor 2(Runx2),ALP,and morphogenetic protein 2(BMP2)].Results Scanning electron microscopy revealed that the PCL-PEG-CGF composite scaffold exhibited a honeycomb-like structure with heterogeneous pore sizes.The composite scaffold exhibited excellent hydrophilicity,as evidenced by a contact angle(θ)approaching 0° within 6 s.Its elastic modulus was measured at(4.590 0±0.149 3)MPa,with compara-ble hydrophilicity,fracture tensile strength,and fracture elongation to PCL-PEG scaffold.The hPDLSCs exhibited signif-icantly improved adhesion to the PCL-PEG-CGF composite scaffold compared with the PCL-PEG scaffold(P<0.01).Ad-ditionally,cell proliferation was markedly improved in all the experimental groups on days 3,5,and 7(P<0.01),and sta-tistically significant differences were found between the PCL-PEG-CGF group and other groups(P<0.01).The PCL-PEG-CGF group showed significantly elevated ALP activity(P<0.05),increased mineralization nodule formation,and upregulated expression of osteogenic-related proteins(Runx2,BMP2 and ALP;P<0.05).Conclusion The PCL-PEG-CGF composite scaffold exhibited excellent mechanical properties and biocompatibility,enhancing the adhesion and pro-liferation of hPDLSCs and promoting their osteogenic differentiation by upregulating osteogenic-related proteins.关键词
血小板浓缩生长因子/聚己内酯—聚乙二醇—血小板浓缩生长因子复合支架/聚己内酯—聚乙二醇支架/人牙周膜干细胞/细胞增殖/成骨分化Key words
concentrated growth factor/polycaprolactone-polyethylene glycol-concentrated growth factor composi-te scaffold/polycaprolactone-polyethylene glycol scaffold/human periodontal ligament stem cells/cell prolifera-tion/osteogenic differentiation分类
医药卫生引用本文复制引用
高丽,赵明月,杨顺,王茹楠,程佳佳,陈广生..聚己内酯—聚乙二醇—血小板浓缩生长因子复合支架的制备及其对人牙周膜干细胞生物学特性的影响[J].华西口腔医学杂志,2025,43(6):819-828,10.基金项目
Science and Technology Fund Project of Guizhou Provincial Health and Health Commission(gzkj2023-202) （gzkj2023-202）
National Natural Science Foundation of China(82060204) （82060204）
Science and Technology Department of Guizhou Pro-vince(ZK[2021]437) 贵州省卫健委科学技术基金(gzwkj2023-202) （ZK[2021]437）
国家自然科学基金(82060204) （82060204）
贵州省科技计划项目[基金黔科合基础—ZK(2021)一般437] （2021）
