华西口腔医学杂志2025，Vol.43Issue(6)：829-836,8.DOI:10.7518/hxkq.2025.2025182
大麻素受体2抑制对口腔黏膜天疱疮棘层松解影响的研究
Cannabinoid receptor 2 inhibition on acantholysis in oral mucosal pemphigus
摘要
Abstract
Objective The aim of this study is to determine the effect of cannabinoid receptor(CB)2 inhibitor on desmoglein 3(DSG3)expression in HaCaT cells co-cultured with pemphigus serum.Methods Immunohistochemical staining was used to compare CB expression in pemphigus patients and normal individuals.Enzyme-linked immunosor-bent assay(ELISA)was employed to quantify the concentration of CB2 in the serum of pemphigus patients and normal individuals.A correlation analysis was performed to examine the relationship between the serum CB2 and DSG of pem-phigus patients.The CCK-8 assay was used to evaluate the inhibitory effect of AM630 on HaCaT cells,and the half-max-imal inhibitory concentration(IC50)value was utilized to determine the experimental concentration.Serum from normal individuals(negative control group)and pemphigus patients(pemphigus group)was co-cultured with HaCaT cells at a 1∶1 ratio.HaCaT cells cultured in complete medium were used as the control group.HaCaT cells in the pemphigus group treated with AM630 were employed as the AM630 group.Real-time polymerase chain reaction(PCR)and Western blot were conducted to assess the expression levels of CB2,DSG3,and β-catenin.Cell dissociation experiments were conducted to evaluate the effect of AM630 on the adhesion of HaCaT cells.Results Immunohistochemistry revealed significant differences in CB2 expression between pemphigus and normal mucosa(P<0.000 1),but no difference was found in CB1 expression.ELISA analysis revealed a statistically significant difference in the expression levels of CB2 in the serum between normal individuals and pemphigus patients(P<0.001).The expression of CB2 in the serum of pem-phigus patients exhibited a significant positive correlation with that of DSG3(r=0.831,P=0.003).The CCK-8 assay indi-cated that the IC50 of AM630 on HaCaT cells was 0.55 μmol/L.Real-time PCR and Western blot showed that the expres-sion levels of CB2 and DSG3 increased in the pemphigus group,while the expression level of β-catenin decreased com-pared with that in the AM630 groups(P<0.05).Conclusion CB2 is highly expressed in oral mucosal pemphigus.AM630 inhibits overexpression of CB2 and DSG3 and underexpression of β-catenin levels,which can provide new thera-peutic targets for pemphigus.关键词
大麻素受体/自身免疫性疾病/天疱疮/桥粒黏蛋白/HaCaT细胞Key words
cannabinoid receptor/autoimmune diseases/pemphigus/desmoglein/HaCaT cell分类
医药卫生引用本文复制引用
刘慧娟,宋鹏,侯亚丽,霍晓,秘利进,刘春艳..大麻素受体2抑制对口腔黏膜天疱疮棘层松解影响的研究[J].华西口腔医学杂志,2025,43(6):829-836,8.基金项目
河北省医学科学研究课题(20241093) Medical Science Research Project of Hebei(20241093) （20241093）
