检验医学与临床2025，Vol.22Issue(22)：3025-3031,7.DOI:10.3969/j.issn.1672-9455.2025.22.001
基于NUSAP1、ADAM17及ERp5表达探讨补阳还五汤对乏氧微环境下肺癌A549细胞的作用机制
Exploration on action mechanism of Buyang Huanwu Decoction on A549 cells in lung cancer under hypoxic microenvironment based on NUSAP1,ADAM17 and ERp5 expression
摘要
Abstract
Objective To explore the action mechanism of Buyang Huanwu Decoction on lung cancer A549 cells under hypoxic microenvironment based on the expression of nucleolar and spindle associated protein 1(NUSAP1),a depolymerin and metalloteinase 17(ADAM17)and endoplasmic reticulum protein 5(ERp5).Methods The A549 cell lines with overexpression of NUSAP1,ADAM17 and ERp5 were constructed and verified by real-time fluorescence quantitative PCR(RT-qPCR).The A549 cells were divided into the control group,hypoxia group,Buyang Huanwu Decoction group,Buyang Huanwu Decoction+pc-NUSAP1 group,Buyang Huanwu Decoction+pc-ADAM17 group and Buyang Huanwu Decoction+pc-ERp5 group.Except for the control group,the other groups were cultured under hypoxia.Except for the control group and hypoxia group,the other groups were treated with Buyang Huanwu Decoction(1 mg/mL).The pcDNA-NUSAP1 plasmid was transfected into the Buyang Huanwu Decoction+pc-NUSAP1 group,the pcDNA-ADAM17 plas-mid was transfected into the Buyang Huanwu Decoction+pc-ADAM17 group,and the pcDNA-ERp5 plasmid was transfected into the Buyang Huanwu Decoction+pc-ERp5 group.The relative mRNA expression levels of NUSAP1,ADAM17 and ERp5 in each group of cells were detected by RT-qPCR.The proliferation,invasion,migration and natural killer(NK)cell immune killing were compared among the groups.The relative protein expression levels of cyclin A2(CCNA2),cyclin D1(CCND1),Vimentin,matrix metalloproteinase-9(MMP-9),MHC class Ⅰ chain-related A(MICA)and MHC class Ⅰ chain-related B(MICB)in each group of cells were detected by Western blotting.Results The relative mRNA expression levels of NUSAP1,ADAM17 and ERp5,as well as the relative protein expression levels of CCNA2,CCND1,Vimentin,MMP-9,MICA and MICB in the hypoxia group were higher than those in the control group(P＜0.05),and the cell proliferation rate,invasion number and scratch healing rate were higher than those in the control group(P＜0.05),while the NK cell killing activity was lower than that in the control group(P＜0.05).The relative mRNA expres-sion levels of NUSAP1,ADAM17 and ERp5,as well as the relative protein expression levels of CCND1,CC-NA2,Vimentin,MMP-9,MICA and MICB in the Buyang Huanwu Decoction group were lower than those in the hypoxia group(P＜0.05),and the cell proliferation rate,invasion number and scratch healing rate were lower than those in the hypoxia group(P＜0.05),while the NK cell killing activity was higher than that in the hypoxia group(P＜0.05).The relative mRNA expression level of NUSAP1,cell proliferation rate,inva-sion number,scratch healing rate and the relative protein expression levels of CCND1,CCNA2,Vimentin and MMP-9 in the Buyang Huanwu Decoction+pc-NUSAP1 group were higher than those in the Buyang Huanwu Decoction group(P＜0.05).The relative mRNA expression level of ADAM17,invasion number,scratch heal-ing rate and the relative protein expression levels of Vimentin,MMP-9,MICA and MICB in the Buyang Huan-wu Decoction+pc-ADAM17 group were higher than those in the Buyang Huanwu Decoction group(P＜0.05),while the NK cell killing activity was lower than that in the Buyang Huanwu Decoction group(P＜0.05).The relative mRNA expression level of ERp5 and the relative protein expression levels of MICA and MICB in the Buyang Huanwu Decoction+pc-ERp5 group were higher than those in the Buyang Huanwu De-coction group(P＜0.05),while the NK cell killing activity was lower than that in the Buyang Huanwu Decoc-tion group(P＜0.05).Conclusion Buyang Huanwu Decoction could inhibit the proliferation and metastasis of A549 cells in hypoxic microenvironment and increase the killing ability of NK cells against tumors,which may be related to the inhibition of the expression of NUSAP1,ADAM17 and ERp5.关键词
肺癌/乏氧微环境/补阳还五汤/仁纺锤体相关蛋白1/解聚素-金属蛋白酶17/内质网蛋白5/细胞转移/免疫逃逸Key words
lung cancer/hypoxic microenvironment/Buyang Huanwu Decoction/nucleolar and spin-dle associated protein 1/a depolymerin and metalloteinase 17/endoplasmic reticulum protein 5/cell me-tastasis/immune escape分类
医药卫生引用本文复制引用
张领兄,贺新玉,路世通,张红..基于NUSAP1、ADAM17及ERp5表达探讨补阳还五汤对乏氧微环境下肺癌A549细胞的作用机制[J].检验医学与临床,2025,22(22):3025-3031,7.基金项目
湖南省中医药科研计划项目(C2023001). （C2023001）
