检验医学与临床2025，Vol.22Issue(22)：3032-3038,7.DOI:10.3969/j.issn.1672-9455.2025.22.002
CKAP2L在吉西他滨耐药膀胱癌细胞中的表达及作用
Expression and role of CKAP2L in gemcitabine-resistant bladder cancer cells
摘要
Abstract
Objective To investigate the expression changes of cytoskeleton-associated protein 2-like(CK-AP2L)in gemcitabine-resistant bladder cancer cells and its effects on the biological behavior of cancer cells and drug sensitivity.Methods The gemcitabine-resistant bladder cancer cell lines T24/GEM and 5637/GEM were established by using the concentration gradient induction method,small interfering RNA was used to knock down CKAP2L gene expression in the respective resistant cells,and T24 and 5637 cells were divided in-to:(1)T24,5637 groups;(2)T24/GEM,5637/GEM groups;(3)NC-T24/GEM,NC-5637/GEM groups;(4)si-T24/GEM,si-5637/GEM groups.The cell proliferation rates were detected by the CCK-8 assay,and the cell migration ability was assessed by the scratch wound healing assay.The CKAP2L mRNA and protein expres-sion levels were detected by the quantitative real-time PCR and Western blotting respectively.The gemcit-abine sensitivity assays were performed on transfected T24/GEM and 5637/GEM cells.Results Compared with the T24 group,the relative mRNA and protein expression levels of CKAP2L in the T24/GEM group were significantly increased(P＜0.05).Compared with the 5637 group,the relative mRNA and protein ex-pression levels of CKAP2L in the 5637/GEM group were significantly increased(P＜0.05).At 48 h,the cell proliferation rate in the si-T24/GEM group was lower than that in the T24 group,T24/GEM group and NC-T24/GEM group(P＜0.05),the cell proliferation rate in the si-5637/GEM group was lower than that in the 5637 group,5637/GEM group and NC-5637/GEM group(P＜0.05).The cell migration rate in the si-T24/GEM group was significantly lower than that in the T24 group,T24/GEM group and NC-T24/GEM group(P＜0.001);the cell migration rate in the si-5637/GEM group was significantly lower than that in the 5637 group,5637/GEM group and NC-5637/GEM group(P＜0.001).At gemcitabine concentration ≥5 μmol/L,the survival rate of si-T24/GEM cell was lower than that of NC-T24/GEM cell(P＜0.05);at gemcitabine concentration ≥2.5 μmol/L,the survival rate of si-5637/GEM cell was lower than that of NC-5637/GEM cell(P＜0.05).The linear regression analysis results showed that the survival rates of cells in NC-T24/GEM,si-T24/GEM,NC-5637/GEM and si-5637/GEM cells were linearly decreased with the gemcitabine concentration increase(P＜0.05),while the descent rate in survival rates of si-T24/GEM cell(R2=0.91,P＜0.05,b=1.71)was significantly faster than that of NC-T24/GEM cell(R2=0.75,P＜0.05,b=0.38),the descent rate in survival rates of si-5637/GEM cell(R2=0.85,P＜0.05,b=1.69)was significantly faster than that of NC-5637/GEM cell(R2=0.93,P＜0.05,b=0.34).Conclusion CKAP2L is highly expressed in gemcitabine-re-sistant bladder cancer T24 and 5637 cells,where it promotes the cell proliferation and migration and reduces the chemosensitivity to gemcitabine.CKAP2L may be an important regulatory molecule in gemcitabine che-moresistance in bladder cancer and a potential therapeutic target.关键词
膀胱癌/细胞骨架相关蛋白2样/吉西他滨/化疗耐药/细胞增殖/细胞迁移Key words
bladder cancer/cytoskeleton-associated protein 2-like/gemcitabine/chemotherapy re-sistance/cell proliferation/cell migration分类
医药卫生引用本文复制引用
胡容珲,郭震,韩露露,张阳阳,刘哓东,王磊..CKAP2L在吉西他滨耐药膀胱癌细胞中的表达及作用[J].检验医学与临床,2025,22(22):3032-3038,7.基金项目
河南省科技攻关计划(联合共建)项目(LHGJ20240835) （联合共建）
河南省科技攻关项目(242102310136). （242102310136）
