农业生物技术学报2025，Vol.33Issue(12)：2571-2580,10.DOI:10.3969/j.issn.1674-7968.2025.12.002
河南省部分小麦新品种(系)HMW-GS组成与1BL/1RS的多样性分析
Identification and Diversity Analysis of HMW-GS Composition and 1BL/1RS in Some New Wheat(Triticum aestivum)Varieties(Lines)in Henan Province
摘要
Abstract
The genetic improvement of wheat(Triticum aestivum)processing quality is a crucial objective in breeding programs,while the composition of high molecular weight glutenin subunits(HMW-GS)and the presence of the 1BL/1RS translocation are 2 significant genetic factors that affecting the processing quality of wheat.In this study SDS-PAGE and acid-polyacrylamide gel electrophoresis(A-PAGE)were employed to systematically characterize the HMW-GS composition and 1BL/1RS translocation background in 151 new wheat varieties(lines)from the 2023 Henan provincial wheat regional trials.The results revealed 12 distinct HMW-GS subunit types,with 3 variants at the Glu-A1 locus,5 at Glu-B1,and 4 at Glu-D1.The frequency of the high-quality subunit 5+10 was relatively low,accounting for only 19.21%.A total of 22 types of HMW-GS subunit combination forms were detected.The average quality score was 6.83 points.The subunit combination with the highest distribution frequency was 1/7+9/2+12(accounting for 27.81%),while the high-quality subunit combination 1/7+8/5+10 only accounted for 8.61%.Additionally,62 wheat varieties(lines)were identified as carrying the 1BL/1RS translocation chromosome,accounting for 41.06%of the tested materials.14 wheat varieties(lines)have been identified,which possess HMW-GS with a score of 10 and were non-1BL/1RS translocation lines.These varieties(lines)hold potential value for breeding strong-gluten wheat.Therefore,the proportion of new wheat varieties(lines)containing high-quality HMW-GS combinations was not high,and the average subunit score was relatively low.However,the distribution frequency of the 1BL/1RS translocation lines had decreased.This study provides crucial genetic background information for wheat breeders in Henan province.The selected non-1BL/1RS translocation lines carrying high-quality subunit combinations could serve as important resources for developing high-yield and high-quality wheat varieties,offering significant guiding value for improving wheat processing quality.关键词
小麦/高分子量谷蛋白亚基(HMW-GS)/1BL/1RS易位系Key words
Wheat/High molecular weight glutenin subunits(HMW-GS)/1BL/1RS translocation分类
农业科技引用本文复制引用
耿惠敏,王孟娜,张大乐..河南省部分小麦新品种(系)HMW-GS组成与1BL/1RS的多样性分析[J].农业生物技术学报,2025,33(12):2571-2580,10.基金项目
河南省科技厅重点研发项目(231111112900 （）
241111110900) （）
