生物医学工程研究2025，Vol.44Issue(5)：303-310,8.DOI:10.19529/j.cnki.1672-6278.2025.05.05
融合显微动态成像与漫射相关光谱的血流多尺度评估方法
A multiscale blood flow assessment method integrating microscopic dynamic imaging and diffuse correlation spectroscopy
摘要
Abstract
To enhance the precise application of diffuse correlation spectroscopy(DCS)in medicine,we combined microscopic red blood cell motion imaging with DCS measurement to explore the connection between microscopic red blood cell motion parameters and macroscopic blood flow parameters.Firstly,the sheep blood samples were systematically analyzed through high-speed microscopic ima-ging and DCS.Then,the red blood cell movement parameters were obtained by continuous microscopic imaging and inter-frame corre-lation analysis of images,the mean square displacement(MSD)and diffusion coefficient of red blood cells were determined.Finally,the tissue blood flow index(BFI)was measured by DCS.The results showed that in the experiments of sheep blood samples at different dilution levels,with the increase of the dilution level,the diffusion coefficient of red blood cells in sheep blood increased.At the same time,the BFI measured by DCS showed an upward trend,indicating that the freedom of movement and diffusion coefficient of red blood cells have been significantly enhanced.Among them,at the moderate dilution level,the red blood cell diffusion coefficient was signifi-cantly positively correlated with the BFI measured by DCS(maximum r=0.63,P<0.001).This method can provide an experimental basis for understanding the mechanism of action between microcirculation disorders and ischemic stroke,cardiovascular diseases and the tumor microenvironment.关键词
漫射相关光谱/红细胞/扩散系数/图像帧间相关性/血流指数Key words
Diffuse correlation spectroscopy/Red blood cells/Diffusion coefficient/Image frame-to-frame correlation/Blood flow index分类
医药卫生引用本文复制引用
吴旭升,王珺琪,徐舒宁,孙王玮,桂志国,尚禹..融合显微动态成像与漫射相关光谱的血流多尺度评估方法[J].生物医学工程研究,2025,44(5):303-310,8.基金项目
国家重点研发计划项目(2023YFE0202800) （2023YFE0202800）
山西省基础研究计划项目(202203021221097,202203021211100) （202203021221097,202203021211100）
中北大学研究生科技立项(20231932,20242027,20242028) （20231932,20242027,20242028）
山西省研究生教育创新项目(2024KY603). （2024KY603）
