生物医学工程研究2025，Vol.44Issue(5)：327-334,8.DOI:10.19529/j.cnki.1672-6278.2025.05.08
全分子量透明质酸钠的经皮渗透特性研究
Study on transdermal permeation characteristics of full molecular weight sodium hyaluronate
王峥 1樊雨梅 1罗小兰 1凌沛学2
作者信息
- 1. 梅晔生物医药股份有限公司,济南 250002
- 2. 梅晔生物医药股份有限公司,济南 250002||山东大学 国家糖工程技术研究中心,青岛 266201
- 折叠
摘要
Abstract
To systematically evaluate the transdermal penetration behavior and effects of full molecular weight sodium hyaluronate(FMW-SH),we took FMW-SH as the research object and determined the molecular weight distribution of FMW-SH by high perform-ance gel chromatography.Using a 3D skin model,we evaluated its transdermal penetration efficiency.Multiple analytical approaches were employed to assess FMW-SH's biological effects,including ELISA for typeⅠcollagen synthesis,scratch assay for keratinocyte mi-gration,immunofluorescence for carboxymethyl lysine(CML)clearance and qPCR for sensitive gene mRNA expression.The results showed that the weight-average molecular weight of FMW-SH was 547.7 kDa,the polydispersion coefficient was 17.679,and the cu-mulative transdermal rate within 24 h reached 26.7％,which was significantly better than that of high molecular weight SH and oligo-meric SH.Cytotoxicity tests confirmed the safety of 0.01％～0.5％FMW-SH concentrations.Significant biological activities were ob-served,0.1％～0.5％FMW-SH enhanced typeⅠcollagen synthesis(P<0.05)and keratinocyte migration rate(P<0.05).0.2％FMW-SH reduced CML expression by 52.92％,outperforming aminoguanidine remarkably.0.0005％FMW-SH effectively suppressed IL-4/IL-13induced upregulation of CAII and NELL2 mRNA(P<0.01).FMW-SH can increase the content of typeⅠcollagen,promote ke-ratinocyte migration,eliminate CML and down-regulate the expression of sensitive genes.关键词
全分子量透明质酸钠/经皮渗透/I型胶原蛋白/细胞划痕/羧甲基赖氨酸Key words
Full molecular weight sodium hyaluronate/Transdermal penetration/Type I collagen/Scratch assay/Carboxymethyl lysine分类
医药卫生引用本文复制引用
王峥,樊雨梅,罗小兰,凌沛学..全分子量透明质酸钠的经皮渗透特性研究[J].生物医学工程研究,2025,44(5):327-334,8.
