生态学杂志2025，Vol.44Issue(11)：3646-3655,10.DOI:10.13292/j.1000-4890.202511.006
生物炭配施肥对杉木林土壤养分含量及微生物群落的影响
Effects of combined application of biochar with fertilizers on soil nutrient contents and microbial communi-ties of Cunninghamia lanceolata plantation
摘要
Abstract
Exploring the changes in soil nutrient contents and microbial communities of forest plantation under dif-ferent combined applications of biochar with fertilizers is of great significance for understanding the mechanism un-derlying soil carbon cycling.A field experiment in a Cunninghamia lanceolata plantation was conducted with five treatments,including control(CK,no materials applied),biochar(B),biochar combined with nitrogen fertilizer(BN),biochar combined with phosphorus fertilizer(BP),biochar combined with bothnitrogen and phosphorus fertilizers(BNP).Soil nutrient contents,microbial biomass,and microbial community structure were measured in different soil layers(0-10,10-20,20-40,and 40-60 cm)in a whole growing season,while the main environ-mental factors influencing soil microbial communities were determined.The results showed that different treatments significantly affected soil nutrient contents and microbial biomass.In the 0-10 cm soil layer,the contents of soil or-ganic carbon(SOC)and total nitrogen(TN)were significantly reduced by 25.3％and 14.9％in the B treatment,20.5％and 13.5％in the BP treatment,and 23.8％and 14.9％in the BNP treatment,respectively.The microbial biomass nitrogen(MBN)in the BP treatment was significantly higher than that in other treatments.In the 10-20 cm layer,SOC contents and microbial biomass carbon(MBC)were significantly lower in BP and BN treatments than CK,respectively.In the 20-40 cm layer,the MBN was significantly lower in B,BP,BP,and BNP treat-ments than in CK,and the microbial biomass phosphorus(MBP)in the BNP treatment was significantly higher than that in the B treatment.In the 40-60 cm layer,the available phosphorus(AP)content was significantly lower in B,BP,BP,and BNP treatments than in CK,while the MBP in the BP treatment was significantly higher than that in other treatments.Additionally,the relative abundance of actinomycetes in 20-40 cm and 40-60 cm soil lay-ers significantly increased in BNP treatment.Our results suggested that SOC played a key role in shaping microbial communities.Biochar application provided carbon sources,enhanced microbial biomass,and regulated microbial community structure.This study provides valuable insights for soil carbon management and sustainable development of C.lanceolata plantations.关键词
生物炭配施肥/土壤养分/微生物生物量/微生物群落结构/化学计量特征Key words
combined application of biochar with fertilizer/soil nutrient/microbial biomass/microbial community structure/stoichiometric characteristics引用本文复制引用
贾志荣,薛旭鹏,范建忠,叶俊成,高歌,葛晓改,李正才..生物炭配施肥对杉木林土壤养分含量及微生物群落的影响[J].生态学杂志,2025,44(11):3646-3655,10.基金项目
"十四五"重点研发子课题(2023YFD2200404-02)资助. （2023YFD2200404-02）
