世界中西医结合杂志2025，Vol.20Issue(10)：1936-1945,10.DOI:10.13935/j.cnki.sjzx.251004
基于生物信息学分析膜性肾病中铁死亡相关基因及中药预测
Ferroptosis-related Genes in Membranous Nephropathy Based on Bioinformatics and Prediction of Chinese Medicine
摘要
Abstract
Objective To identify ferroptosis-related genes(FRGs)in membranous nephropathy(MN)using bioinformatics and to predict potential therapeutic Chinese medicines and their core compounds.Methods Gene expres-sion datasets GSE200828 and GSE108109 were downloaded from the GEO database,and FRGs were obtained from the FerrDb public database.The intersection of the two datasets was defined as MN-FRGs.Enrichment analysis of MN-FRGs was performed,and a protein-protein interaction network was constructed using the STRING database.Differential gene expression was validated using the Nephroseq v5 database.The identified differential genes were imported into the Coremine database to predict relevant Chinese medicines.A core Chinese medicine-compound network was constructed u-sing Cytoscape 3.9.0,and topological analysis was conducted.Molecular docking between core compounds and key targets was performed using AutoDock Tools.Results Fifteen MN-FRGs were identified.KEGG pathway analysis indicated that MN-FRGs were primarily involved in the advanced glycation end product-receptor for AGE(AGE-RAGE)signaling pathway in chemical carcinogenesis-reactive oxygen species diabetic complications,phosphatidylinositol 3-kinase-pro-tein kinase B(PI3K-Akt)signaling pathway,and fluid shear stress-atherosclerosis pathway.Validation with Nephroseq v5 revealed significantly decreased expression of albumin(ALB),recombinant nicotinamide adenine dinucleotide phosphate oxidase 4(NOX4),phosphoenolpyruvate carboxykinase 2(PCK2),phosphoserine aminotransferase 1(PSAT1),metallothio-nein 1G(MT1G),DNA damage-inducible transcript 4(DDIT4).Using MN-FRGs as targets,42 Chinese medicines and 572 related compounds were predicted.Molecular docking results showed good binding affinity between the compounds and key targets,with docking energies all＜5 kcal·mol-1.Conclusion This study identified FRGs in MN,their associated signaling pathways,and potential Chinese medicines and core compounds.These findings provide a foundation and direction for further research on the mechanisms by which Chinese medicines may intervene in ferroptosis.关键词
生物信息学/膜性肾病/铁死亡/差异基因/中药Key words
Bioinformatics/Membranous Nephropathy/Ferroptosis/Differential Gene/Chinese Medicine分类
医药卫生引用本文复制引用
石秀杰,常美莹,张昱,史振伟..基于生物信息学分析膜性肾病中铁死亡相关基因及中药预测[J].世界中西医结合杂志,2025,20(10):1936-1945,10.基金项目
国家自然科学基金项目(82405282) （82405282）
北京市自然科学基金面上项目(7232315) （7232315）
