中国土壤与肥料Issue(10)：67-83,98,18.DOI:10.11838/sfsc.1673-6257.25048
杧果树滴水线内土壤真菌和细菌菌落结构特征分析
Culture structure characteristics analysis of soil fungal and bacterial in the drip line of mango tree
摘要
Abstract
Mangifera indica Linn.,a highly significant economic crop thriving in tropical and subtropical zones,is subject to the impact of numerous factors.Soil microorganisms play an indispensable role in regulating the growth of Mangifera indica Linn.Soil samples from different depths were collected and analyzed by Illumina sequencing and bioinformatics to explore the structure of fungal and bacterial colonies in the soil of mango trees along the drip line.The results showed that,(1)The structural characteristics of soil fungal colonies:The diversity of fungal colonies was the most abundant in the soil depth of 0-20 cm,while the diversity of fungal colonies was relatively low and the distribution was uneven in the soil depth of 20-40 cm.There were 1883 species of soil fungi in the three samples,with the most abundant species in the 0-20 cm soil sample.The fungi from the three samples belonged to 13 phyla,and the dominant phyla were Ascomycota,Basidiomycota and Mortierellomycota,and so on.The three samples contained 453 genera,including Mortierella,Talaromyces,Trichodermas,and so on.Sixty-seven species from the three samples could explain the differences among the groups.892 kinds of enzymes were obtained from the three samples,and the pathways with higher relative abundance were aerobic respiration I(cytochrome c),aerobic respirationⅡ(cytochrome c),and palmitate biosynthesis I.(2)The structure of bacterial colonies:Similar to the diversity of fungal colonies,soil samples of 0-20 cm had the highest bacterial colony richness.A total of 10903 species of soil bacteria were identified in the drip line of mango trees.The distribution of total and unique bacteria in different samples showed the order of 0-20 cm>40-60 cm>20-40 cm.A total of 41 phyla was identified in the three samples,including Proteobacteria,Acidobacteriota,and Firmicutes.All the bacteria in the three samples were classified into 907 genera,including Bacillus,Flavobacterium and Pseudomonas,and so on.These 95 species from the three samples could explain the differences among the groups.A total of 4359 proteins were detected,which were grouped into 23 categories,covering a variety of important physiological function categories such as amino acid transport and metabolism,energy production and conversion,and extracellular structure.These 2326 enzymes were detected in all three samples,including DNA-directed DNA polymerase,ubiquinone reductase[H(+)-transporting]DNA helicase,Histidine kinase,Peptidylprolyl isomerase,and other enzymes that were essential for bacterial life activities.There were 439 kinds of pathways,and their abundance values ranged from 0.03 to 127225.30.The highest relative abundance were aerobic respiration I(cytochrome c),isobutanol production by pyruvate fermentation(engineering),L-isoleucine biosynthesisⅡ.In conclusion,there were abundant fungi and bacteria in the surface soil of mango trees in the drip line.There were significant differences in the relative abundance of bacteria with different functions and specific changing patterns in different samples.In addition,the relative abundance of specific metabolic pathways varied among samples,which provided important information for understanding the metabolic characteristics of soil fungi and bacteria in the drip line of mango tree.关键词
杧果树/高通量测序/土壤真菌/土壤细菌/菌落特征Key words
mango tree/high throughput sequencing/soil fungal/soil bacterial/culture characteristics分类
农业科技引用本文复制引用
LIU Rong,ZHANG Zheng-xue,LEI Yue,HUANG Hai,LIU Fan-zhi,DANG Zhi-guo..杧果树滴水线内土壤真菌和细菌菌落结构特征分析[J].中国土壤与肥料,2025,(10):67-83,98,18.基金项目
国家香蕉产业技术体系(CARS-31) （CARS-31）
贵州省农业科学院项目(黔农科种质资源[2023]08号). （黔农科种质资源[2023]08号）
