中国土壤与肥料Issue(10)：84-91,8.DOI:10.11838/sfsc.1673-6257.25029
不同种植年限对设施蔬菜地土壤细菌群落的影响
Effects of different planting years on soil microbial community in vegetable plots
摘要
Abstract
s:Long-term cultivation in greenhouse facilities could lead to soil compaction,soil acidification,and soil salinization,which in turn caused imbalances in soil bacterial communities and severely restricted the sustainable development of facility vegetables.The high-throughput sequencing technology and other methods were utilized to investigate the effects of different planting years on soil bacterial communities and soil chemical properties in facility vegetable fields,and to explore the correlations between soil chemical factors and bacterial community changes.The results showed that as the planting years increased,soil pH decreased from 5.85 to 5.11,total nitrogen increased from 1.29 g/kg to 1.68 g/kg,available nitrogen increased from 120.22 mg/kg to 222.40 mg/kg(P<0.05),and total salinity increased from 1.97%to 2.85%-3.27%.After 20 years of planting,bacterial α-diversity significantly decreased(P<0.05),and PERMANOVA analysis indicated significant changes in bacterial community structure(P<0.01).Among the top 10 phyla and genera,the content of Proteobacteria decreased to the lowest level of 20.69%,while the contents of Firmicutes and Chloroflexi increased by 3.21%and 22.14%,respectively,reaching 4.40%and 30.40%.The relative abundances of Sphingomonas and Bacillus decreased by 3.66%and 1.94%,respectively(P<0.05).Canonical correlation analysis indicated that pH and available nitrogen were the main environmental factors affecting bacterial community structure(P=0.011,P=0.036).With the extension of planting time(>10 years),soil acidification and nitrogen accumulation occurred,significantly reducing the diversity of bacterial communities,altering the structure of bacterial communities,and subsequently causing an imbalance in soil microecology,which could restrict the sustainable utilization of facility vegetable land.关键词
细菌群落结构/种植年限/设施大棚/细菌多样性/土壤酸化Key words
bacterial community structure/years of planting/facility greenhouses/soil physicochemical properties/soil acidification引用本文复制引用
曾德胜,刘加强,李宇慧,刘艺璇,马超,王擎运..不同种植年限对设施蔬菜地土壤细菌群落的影响[J].中国土壤与肥料,2025,(10):84-91,8.基金项目
安徽省重点研究与开发项目(2023n06020056) （2023n06020056）
国家自然科学基金项目(32071628) （32071628）
安徽省高校科学基金(2024AH020002). （2024AH020002）
