首页|期刊导航|微生物（英文）|A CRISPR-nonhomologous end-joining-based strategy for rapid and efficient gene disruption in Mycobacterium abscessus

A CRISPR-nonhomologous end-joining-based strategy for rapid and efficient gene disruption in Mycobacterium abscessus

Sanshan Zeng Jie Ding Xinyue Wang Jinxing Hu Shuai Wang Tianyu Zhang Yanan Ju Md Shah Alam Ziwen Lu H.M.Adnan Hameed Lijie Li Xirong Tian Cuiting Fang Xiange Fang

微生物（英文）2025，Vol.4Issue(2)：169-180,12.

微生物（英文）2025，Vol.4Issue(2)：169-180,12.DOI:10.1002/mlf2.70007

A CRISPR-nonhomologous end-joining-based strategy for rapid and efficient gene disruption in Mycobacterium abscessus

Sanshan Zeng ¹Jie Ding ²Xinyue Wang ³Jinxing Hu ⁴Shuai Wang ⁵Tianyu Zhang ⁶Yanan Ju ⁷Md Shah Alam ¹Ziwen Lu ¹H.M.Adnan Hameed ⁸Lijie Li ⁷Xirong Tian ¹Cuiting Fang ⁹Xiange Fang¹

作者信息

1. State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||China-New Zealand Joint Laboratory on Biomedicine and Health,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||University of Chinese Academy of Sciences,Beijing,China
2. State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||China-New Zealand Joint Laboratory on Biomedicine and Health,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||Institute of Physical Science and Information Technology,Anhui University,Hefei,China
3. State Key Laboratory of Respiratory Disease,Guangzhou Chest Hospital,Guangzhou,China||Guangzhou National Laboratory,Guangzhou,China
4. State Key Laboratory of Respiratory Disease,Guangzhou Chest Hospital,Guangzhou,China
5. State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||China-New Zealand Joint Laboratory on Biomedicine and Health,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||State Key Laboratory of Respiratory Disease,Guangzhou Chest Hospital,Guangzhou,China
6. State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||China-New Zealand Joint Laboratory on Biomedicine and Health,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||University of Chinese Academy of Sciences,Beijing,China||School of Basic Medical Sciences,Division of Life Science and Medicine,University of Science and Technology of China,Hefei,China||State Key Laboratory of Respiratory Disease,Guangzhou Chest Hospital,Guangzhou,China||Institute of Physical Science and Information Technology,Anhui University,Hefei,China||Guangzhou National Laboratory,Guangzhou,China
7. State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||China-New Zealand Joint Laboratory on Biomedicine and Health,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||School of Basic Medical Sciences,Division of Life Science and Medicine,University of Science and Technology of China,Hefei,China
8. State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||China-New Zealand Joint Laboratory on Biomedicine and Health,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China
9. State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||China-New Zealand Joint Laboratory on Biomedicine and Health,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China||University of Chinese Academy of Sciences,Beijing,China||State Key Laboratory of Respiratory Disease,Guangzhou Chest Hospital,Guangzhou,China
折叠

摘要

关键词

CRISPR-Cas12a/DSB repair/Mycobacterium abscessus/NHEJ/NrgA

Key words

CRISPR-Cas12a/DSB repair/Mycobacterium abscessus/NHEJ/NrgA

引用本文复制引用

Sanshan Zeng,Jie Ding,Xinyue Wang,Jinxing Hu,Shuai Wang,Tianyu Zhang,Yanan Ju,Md Shah Alam,Ziwen Lu,H.M.Adnan Hameed,Lijie Li,Xirong Tian,Cuiting Fang,Xiange Fang..A CRISPR-nonhomologous end-joining-based strategy for rapid and efficient gene disruption in Mycobacterium abscessus[J].微生物（英文）,2025,4(2):169-180,12.

基金项目

This study was supported by the National Key R&D Program of China(2021YFA1300900 and 2022YFE0210600),the National Natural Science Foundation of China(21920102003),the Guangdong Province Grant for Belt and Road Joint Laboratory(2022B1212050004),the State Key Lab of Respiratory Disease,the Postdoctoral Fellowship Program of CPSF(GZC20232688),the Guangzhou Institute of Respiratory Diseases,First Affiliated Hospital of Guangzhou Medical University(SKLRD-Z-202301,SKLRD-Z-202412),the Guangzhou Science and Technology(2024A04J4273),and the Chinese Academy of Sciences,Presi-dent's International Fellowship Initiative(2023VBC0015).We also extend our sincere appreciation to Professor Yichen Sun from the Chinese Academy of Medical Sciences and Peking Union Medical College for generously providing the pJV53-Cpf1 and pCR-Zeo plasmids for our study. （2021YFA1300900 and 2022YFE0210600）

微生物（英文）

访问量1

下载量0

段落导航