应用化学2025，Vol.42Issue(11)：1539-1549,11.DOI:10.19894/j.issn.1000-0518.250285
基于UPLC-Q Exactive Plus-MS技术的益肾乌发口服液化学成分分析
Analysis of Chemical Constituents of Yishen Wufa Oral Liquid Based on Ultra Performance Liquid Chromatography Quadrupole-Orbitrap Q Exactive Plus Mass Spectrometer
摘要
Abstract
To qualitatively characterize the chemical constituents in Yishen Wufa Oral Liquid by UPLC-Q Exactive Plus-MS.The analysis was performed on Waters Hypersil GOLD C18(150 mm×2.1 mm,2.5 μm)with the mobile phase of 0.1%formic acid solution(B)and acetonitrile(A)for gradient elution.The electrospray ionization(ESI)was employed,and the data was collected in positive and negative ion full-scan modes with a scanning range.The local database for chemical constituents of individual Chinese medicinal herbs in Yishen Wufa Oral Liquid was established using Xcalibur 4.1 software.And chemical composition characterization was carried out by combining information from the literature and reference substances.A total of 55 chemical constituents were tentatively identified,including 3 amino acids and derivatives,10 terpenoids,3 steroid hormone,4 organic acids,4 coumarins,18 flavonoids,8 anthraquinone and 5 other compounds.The established UPLC-Q Exactive Plus-MS can quickly and comprehensively analyze the chemical composition of Yishen Wufa Oral Liquid,which can provide a scientific basis for the researches of its material basis and quality control.关键词
益肾乌发口服液/高效液相色谱串联四极杆静电场轨道阱质谱/化学成分/定性分析Key words
Yishen Wufa Oral Liquid/UPLC-Q Exactive Plus-MS/Chemical constituents/Qualitative analysis分类
化学化工引用本文复制引用
赫玉芳,李豪,赵建慧,马洋,司英奇,南敏伦,赵昱玮..基于UPLC-Q Exactive Plus-MS技术的益肾乌发口服液化学成分分析[J].应用化学,2025,42(11):1539-1549,11.基金项目
吉林省科技发展计划项目(No.20230401060YY)资助 Supported by Jilin Province Science and Technology Development Plan Project(No.20230401060YY) （No.20230401060YY）
