中国实验动物学报2025，Vol.33Issue(10)：1473-1482,10.DOI:10.3969/j.issn.1005-4847.2025.10.008
2型糖尿病食蟹猴焦虑抑郁样行为评价研究
Evaluation of anxiety-and depression-like behaviors in Cynomolgus monkeys with type 2 diabetes
摘要
Abstract
Objective To investigate anxiety-and depression-like behaviors in Cynomolgus monkeys with type 2 diabetes mellitus(T2DM),and to explore their correlations with biochemical parameters.Methods Cynomolgus monkeys were divided into a T2DM group(fasting plasma glucose(FPG)≥ 5.6 mmol/L)and control group(FPG＜4.2 mmol/L)(n=3 per group).Age,sex,body mass,body mass index(BMI),FPG,blood lipids,atherosclerosis index(AI),and tumor necrosis factor-α(TNF-α)levels were measured in both groups.Anxiety-like behaviors were assessed using the human intrude test(HIT)and depression-like behaviors were evaluated using the apathy feeding test(AFT).Relationships between behavioral parameters and biochemical/inflammatory markers were evaluated using Pearson's correlation analysis.Results(1)FPG,AI,and TNF-αlevels were significantly elevated while high-density lipoprotein cholesterol levels were reduced in the T2DM group compared with the control group(P＜0.05).(2)Regarding the HIT result,the durations of anxious(stare phase)and back-of-cage(stare phase)behaviors were higher in the T2DM group than in the control group(P＜0.05),while the durations of locomotor behaviors(baseline phase,profile phase,back phase)were significantly shorter(P＜0.05).(3)The AFT revealed that food retrieval latency was significantly delayed in the T2DM group(P＜0.05).Pearson's analysis identified a positive correlation between back-of-cage(stare phase)duration and AI(r=0.828,P=0.042),while locomotion during the back phase showed a negative corr-elation with AI(r=-0.842,P=0.035).Conclusions Cynomolgus monkeys with T2DM display distinct anxiety-and depression-like behavioral phenotypes,with a significant association between the AI and anxiety-related behaviors.These findings provide novel insights into the pathophysiological mechanisms linking T2DM with neuropsychiatric comorbidities.关键词
2型糖尿病/焦虑/抑郁/行为学评价/食蟹猴Key words
type 2 diabetes mellitus/anxiety/depression/behavioral test/Cynomolgus monkeys分类
生物科学引用本文复制引用
张琳,宋顺杰,严易,严瑾,杨勇,黎寿岩,岳峰..2型糖尿病食蟹猴焦虑抑郁样行为评价研究[J].中国实验动物学报,2025,33(10):1473-1482,10.基金项目
科技创新2030项目(2021ZD0200900),海南省自然科学基金高层次人才项目(22RC588).Funded by the Scientific and Technological Innovation 2030(2021ZD0200900),High-Level Talents Project of Hainan Natural Science Foundation(22RC588). （2021ZD0200900）
