中国实验动物学报2025，Vol.33Issue(10)：1504-1512,9.DOI:10.3969/j.issn.1005-4847.2025.10.011
基于尾静脉高压注射-转座子介导的原发性小鼠肝癌模型方法及其应用进展
Primary mouse liver cancer model development using hydrodynamic tail vein injection combined with transposon system:progress in its application
摘要
Abstract
Mice have been widely used in the study of primary liver cancer owing to the close similarity of its genome to that of humans,its strong reproductive ability,the low cost of model construction,and the ease of genetic manipulation,including molecular mechanisms of pathogenesis and potential drug targets.Traditional animal models are increasingly falling short of meeting the needs of precision medicine research because of their inability to reproduce tumor microenvironment interactions and control the specificity of molecular subtypes.This study systematically compared the technical advantages of tail vein high-pressure injection,combined with transposon system(HTVI-TS),with traditional models in liver cancer research,and focused on the application value of the HTVI-TS model in the mechanism study of tumorigenesis and development,immunotherapy response prediction,and individualized evaluation of targeted drugs.This report presents a new research platform for precise diagnosis and treatment of primary liver cancer by simulating the heterogeneous evolution process of the cancer.The findings provide a theoretical basis for optimizing the selection of preclinical research models for liver cancer;the expansion potential of this technology in liver cancer research is outlined.关键词
肝癌/小鼠模型/尾静脉注射/转座子Key words
liver cancer/mice HCC model/tail vein injection/transposon分类
生物科学引用本文复制引用
强正华,洪智轩,骆境怡,何小柏,陈林洁..基于尾静脉高压注射-转座子介导的原发性小鼠肝癌模型方法及其应用进展[J].中国实验动物学报,2025,33(10):1504-1512,9.基金项目
浙江省自然科学基金项目(LMS25H160004),浙江省医药卫生科技项目(2023KY650),大学生创新创业训练计划项目(S202513023049).Funded by Zhejiang Natural Science Foundation Project(LMS25H160004),Zhejiang Medical and Health Science and Technology Project(2023KY650),College Students' Innovation and Entrepreneurship Training Program Project(S202513023049). （LMS25H160004）
