中国肿瘤生物治疗杂志2025，Vol.32Issue(11)：1128-1135,8.DOI:10.3872/j.issn.1007-385x.2025.11.004
可捕获抗原的负载ICG的纳米胶束通过靶向淋巴结诱导肿瘤原位疫苗效应
Antigen-capturing ICG-loaded nanomicelles induce in situ tumor vaccine effects through targeting lymph nodes
摘要
Abstract
Objective:To prepare and characterize indocyanine green(ICG)-loaded F127 nanomicelles(F127-ICG)and explore their anti-tumor effects through their photothermal effect,antigen-capturing capability,and lymph node(LN)-targeting properties.Methods:F127-ICG was prepared using the thin-film hydration method.The particle size and Zeta potential were measured using a Malvern particle size analyzer.The absorption and fluorescence spectra were determined using ultraviolet-visible and fluorescence spectrophotometry,respectively.The antigen-capturing capability of F127-ICG was analyzed by comparing the basic properties and protein content changes of F127 nanomicelles before and after incubation with tumor cell lysates.The photothermal cytotoxicity of F127-ICG against breast cancer cells(4T1)was evaluated using a Calcein-AM/PI double-staining assay.Dye-labeled F127 nanomicelles were subcutaneously injected into mice to establish a lymphatic drainage model.The LN targeting ability of F127 nanomicelles was detected using in vivo small animal imaging,and the accumulation and penetration of F127 nanomicelles in the inguinal and axillary lymph nodes of mice were detected by ex vivo organ imaging.A bilateral breast cancer model was established on the back of the mouse,and F127-ICG was injected intratumorally for photothermal therapy(PTT).Meanwhile,the growth of the contralateral tumor was monitored.Results:The F127-ICG nanomicelles were successfully prepared,with a mean particle size of(19.41±0.49)nm and an average Zeta potential of-(2.78±0.36)mV.After co-incubation of F127-ICG with tumor antigens,the particle size,Zeta potential negativity,and protein content all increased(P<0.05).The results of the Calcein-AM/PI double staining assay revealed that F127-ICG could effectively exert a photothermal effect to mediate the killing of 4T1 cells.In vivo imaging results revealed that F127 nanomicelles can effectively target LN.In animal experiments,the contralateral tumor volume in the F127-ICG+Laser group was smaller than that in the PBS and F127-ICG groups(P<0.05).Conclusion:F127-ICG-mediated photothermal effects can effectively ablate primary tumors and capture released tumor-specific antigens and then migrate to local LN,which promotes anti-tumor immune response,inhibits distant tumor growth,and enhances in situ vaccine efficacy.关键词
肿瘤疫苗/纳米胶束/抗原捕获/淋巴结/光热治疗Key words
tumor vaccine/nanomicelle/antigen capture/lymph node/photothermal therapy分类
医药卫生引用本文复制引用
程文静,张澄蔚,宋银宏,余祥..可捕获抗原的负载ICG的纳米胶束通过靶向淋巴结诱导肿瘤原位疫苗效应[J].中国肿瘤生物治疗杂志,2025,32(11):1128-1135,8.基金项目
肿瘤微环境与免疫治疗湖北省重点实验室开放基金(No.2023KZL024) （No.2023KZL024）
