中国癌症防治杂志2025，Vol.17Issue(5)：613-618,6.DOI:10.3969/j.issn.1674-5671.2025.05.13
C.leadbetteri对舌鳞状细胞癌细胞系Cal27增殖、侵袭和迁移能力的影响
Impact of C.leadbetteri on the proliferation,invasion and migration of Cal27 tongue squamous cell carcinoma cell line
摘要
Abstract
Objective To investigate the impact of Capnocytophaga leadbetteri(C.leadbetteri),isolated from tissues of patients with tongue squamous cell carcinoma(TSCC),on the proliferation,invasion,and migration of TSCC Cal27 cell line.Methods The C.leadbetteri strain n95,previously isolated and cultured by our research group from the tumor tissue of TSCC patients,were identified through revival culture,colony morphology observation,Gram staining,16S rRNA sequencing analysis,and phylogenetic tree construction.Cal27 cells were infected with C.leadbetteri n95 suspension(CAL27-C.L group),while uninfected Cal27 cells(CAL27 group)served as the control.Adhesion counts of cells and bacterial were recorded 2 h post-infection.The proliferative capacity of Cal27 cells at different multiplicity of infection(MOI=O,10,25)and time points(0,24,48,72 h)was assessed using the CCK-8 assay.Transwell assays were employed to evaluate the migration and invasion capabilities of cells.Results Based on 16S rRNA sequences alignment,which exhibits a 99.63％sequence identity with the reference strain H6253,and Neighbour-Joining phylogenetic tree,strain n95 was identified as C.leadbetteri.Cell adhesion assays demonstrated that the adhesion index of C.leadbetteri n95 to Cal27 cells was 1.62±0.57.The CCK-8 assay results demonstrated that the proliferation activity of Cal27 cells was significantly enhanced(P＜0.05)after 24 h of infection with C.leadbetteri n95 at an MOI of 10∶1.Transwell assays revealed that the invasion and migration capabilities of the CAL27-C.L group were significantly enhanced compared to the CAL27 group(P＜0.01).Conclusion C.leadbetteri n95,isolated from TSCC,effectively adheres to Cal27 cells and markedly boots their proliferation,migratory and invasive capacities under in vitro.关键词
舌鳞状细胞癌/C.leadbetteri/增殖/迁移/侵袭Key words
Tongue squamous cell carcinoma/C.leadbetteri/Proliferation/Migration/Invasion分类
医药卫生引用本文复制引用
于苗苗,周建华,张春艳,袁荣涛,关智辉,巩陆阳,王奇民,陈行,郭庆圆,王莉莉..C.leadbetteri对舌鳞状细胞癌细胞系Cal27增殖、侵袭和迁移能力的影响[J].中国癌症防治杂志,2025,17(5):613-618,6.基金项目
山东省医药卫生科技项目(202408020667) （202408020667）
