中国癌症防治杂志2025，Vol.17Issue(5)：619-626,8.DOI:10.3969/j.issn.1674-5671.2025.05.14
葛根素对鼻咽癌细胞恶性生物学行为的影响及其分子机制
Impact of puerarin on malignant biological behaviour of nasopharyngeal carcinoma cells and its underlying molecular mechanisms
摘要
Abstract
Objective To investigate the impact of puerarin on the proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT)in nasopharyngeal carcinoma cells,as well as to elucidate its potential mechanism.Methods Nasopharyngeal carci-noma 5-8F and HK-1 cells were exposed to varying concentrations of puerarin to identify the optimal concentration for intervention using the CCK-8 assay.The cells were divided into the negative control group and the puerarin group,receiving their respective treatments.Cycle progression was assessed by flow cytometry,cell migration was assessed via a scratch assay,and cell invasion was evaluated using a Transwell assay.Western blot and immunofluorescence assays were conducted to quantify the expression of proteins associated with the EMT process and the PI3K/AKT/mTOR signaling pathway.Furthermore,5-8F and HK-1 cells were co-treated with the PI3K activator 740Y-P and puerarin,followed by Western blot analysis to detect the expression of proteins related to the PI3K/AKT/mTOR signaling pathway.The effects of puerarin on tumour cell proliferation were validated via CCK-8 assays,and clonogenic assays.Results The viability of nasopharyngeal carcinoma 5-8F and HK-1 cells was significantly reduced(all P＜0.0001)upon exposure to varying concentrations(200-1,600 μmol/L)of puerarin,exhibiting a dose-dependent effect.Compared with the control group,puerarin at concentrations of 800 μmol/L and 1,200 μmol/L significantly suppressed the proliferation,migration,and invasion capabilities of naso-pharyngeal carcinoma 5-8F and HK-1 cells(all P＜0.001).Additionally,puerarin treatment resulted in cell cycle at the G0/G1 phase(all P＜0.05),enhanced the protein expression levels of the epithelial marker E-cadherin,and reduced the protein expression levels of the mesenchymal markers N-cadherin and vimentin.Puerarin downregulated the expression of PI3K,AKT,mTOR and their phosphorylated forms,whereas the PI3K activator 740Y-P reversed the puerarin-induced changes in phosphorylation levels of PI3K,AKT and mTOR,and attenuated the inhibitory effect of puerarin on nasopharyngeal carcinoma cell proliferation(all P＜0.01).Conclusions Puerarin inhibits the proliferation,migration,invasion,and EMT in nasopharyngeal carcinoma 5-8F and HK-1 cell lines,possibly by modulating the PI3K/AKT/mTOR signaling pathway.关键词
鼻咽癌/葛根素/侵袭/迁移/上皮-间质转化Key words
Nasopharyngeal carcinoma/Puerarin/Invasion/Migration/Epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
林寒鑫,罗雪文,张春雨,刘兴慧,赵伟..葛根素对鼻咽癌细胞恶性生物学行为的影响及其分子机制[J].中国癌症防治杂志,2025,17(5):619-626,8.基金项目
广西中医药适宜技术开发与推广项目(GZSY22-70) （GZSY22-70）
