中国肿瘤生物治疗杂志2025，Vol.32Issue(11)：1159-1168,10.DOI:10.3872/j.issn.1007-385x.2025.11.008
通过单细胞测序揭示结直肠癌浸润性B细胞的异质性及其预后价值
Single-cell RNA sequencing unravels heterogeneity and prognostic significance of tumor-infiltrating B cells in colorectal cancer
和倩 1王志刚1
作者信息
- 1. 上海交通大学医学院附属第六人民医院 普通外科,上海 200233
- 折叠
摘要
Abstract
Objective:To characterize the heterogeneity of B cells in the colorectal cancer(CRC)tumor microenvironment(TME)through single-cell RNA sequencing(scRNA-seq),thereby providing a theoretical foundation for B cell-based prognostic assessment and immunotherapy.Methods:Fresh surgical specimens from patients with primary colorectal cancer(n=36)were collected and subjected to scRNA-seq.Cell clustering was performed using Seurat(v5.0).Cell differentiation trajectories were analyzed with Monocle3(v1.3)and scVelo(v0.3.0).Gene Set Enrichment Analysis(GSEA)was employed to identify relevant functional pathways,and the gene regulatory network was constructed using pySCENIC(v0.11.2).Results:11 functional subtypes were identified from 24 652 B cells and 49 138 plasma cells.Pseudotime analysis revealed a differentiation trajectory of B cells from a naive to a tissue-resident state.Stress-adapted B cells were enriched in tumor tissues(10.39%),exhibiting significant activation of the NOD-like receptor signaling pathway and antigen processing and presentation.Tissue-resident B cells accounted for 9.09%of tumor-infiltrating B cells and showed enhanced C-type lectin receptor signaling and endocytosis.Migratory B cells were least abundant in stage Ⅱ tumors(16.42%),with significant enrichment of metabolic pathways such as oxidative phosphorylation.Plasma cells were terminally differentiated,with IgG-type cells predominating in tumor tissues(20.68%).Conclusion:Stress-adapted and tissue-resident B cells may promote immune escape,while migratory B cells potentially exert anti-tumor effects.IgG-type plasma cells appear to be associated with immunosuppression.关键词
结直肠癌/单细胞RNA测序/肿瘤浸润性B细胞/肿瘤微环境/预后/异质性Key words
colorectal cancer(CRC)/single-cell RNA sequencing(scRNA-seq)/tumor-infiltrating B cell/tumor microenvironment(TME)/prognosis/heterogeneity分类
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和倩,王志刚..通过单细胞测序揭示结直肠癌浸润性B细胞的异质性及其预后价值[J].中国肿瘤生物治疗杂志,2025,32(11):1159-1168,10.
