中国中医药信息杂志2025，Vol.32Issue(12)：30-38,9.DOI:10.19879/j.cnki.1005-5304.202505257
基于网络药理学及实验验证探究痰瘀同治优化方治疗动脉粥样硬化作用机制
Exploration on Mechanism of Tanyu Tongzhi Optimized Prescription in Anti-atherosclerosis Based on Network Pharmacology and Experimental Validation
摘要
Abstract
Objective To explore the pharmacodynamic material basis and mechanism of Tanyu Tongzhi Optimized Prescription in the treatment of atherosclerosis(AS)using network pharmacology and animal experiments.Methods UPLC-Q-TRAP-MS/MS was used to identify the blood-entering components of Tanyu Tongzhi Optimized Prescription and conduct network pharmacological analysis.Important components and their core targets for the treatment of AS were screened,and molecular docking was conducted.The AS model mice were constructed and treated with Tanyu Tongzhi Optimized Prescription.HE staining and oil red O staining were used to observe aortic tissue morphology.The blood lipid status was detected by an automatic blood biochemical analyzer.The contents of serum inflammatory factors were detected by ELISA,and the mRNA expressions of aortic core targets were detected by RT-qPCR.Results It was identified and predicted that 30 blood-entering components of Tanyu Tongzhi Optimized Prescription might exert therapeutic effects on AS by regulating core targets such as IL6,TNF,IL1B,AKT1 and NFKB1,and regulating the TNF signaling pathway,Toll-like receptor signaling pathway,PI3K-Akt signaling pathway,etc.Animal experiments showed that Tanyu Tongzhi Optimized Prescription could reduce aortic plaque area and inflammatory cell infiltration,alleviate lipid deposition and vascular stenosis,improve blood lipid levels,and significantly reduce the serum contents of TNF-α,IL-1β,and mRNA expressions of TNF-α,IL-6,AKT1 and NF-κB in aortic tissue(P<0.05,P<0.01).Conclusion The effect of Tanyu Tongzhi Optimized Prescription may regulate TNF,Toll-like receptor and other signaling pathways by acting on core targets such as IL6,TNF,IL1B,and inhibit inflammatory response,so as to treat AS.关键词
痰瘀同治优化方:动脉粥样硬化/网络药理学/分子对接/实验验证Key words
Tanyu Tongzhi Optimized Prescription/atherosclerosis/network pharmacology/molecular docking/experimental verification分类
医药卫生引用本文复制引用
周凌风,李盈盈,唐丹丽,张华敏,李想..基于网络药理学及实验验证探究痰瘀同治优化方治疗动脉粥样硬化作用机制[J].中国中医药信息杂志,2025,32(12):30-38,9.基金项目
国家自然科学基金(81973722、82374182) （81973722、82374182）
中国中医科学院科技创新工程(KYG2024-04) （KYG2024-04）
中央级公益性科研院所基本科研业务费专项资金(YPX202305) （YPX202305）
