Therapeutic potential of Traditional Chinese Medicine Yisui Shengxuepills(益髓生血丸)to inhibit hypoxia-inducible factor1alpha and general control nonderepressible 2 to regulate the post chemotherapy immune response...
QI Yafeng ZHANG Liying ZHANG Zhiming LIU Yu LIU Yeyuan LI Yangyang ZHANG Shangzu CHEN Yaping XU Qian HAO Guoxiong LIU Yongqi
中医杂志（英文版）2025，Vol.45Issue(5)：1087-1097,11.
中医杂志（英文版）2025，Vol.45Issue(5)：1087-1097,11.DOI:10.19852/j.cnki.jtcm.2025.05.015
Therapeutic potential of Traditional Chinese Medicine Yisui Shengxuepills(益髓生血丸)to inhibit hypoxia-inducible factor1alpha and general control nonderepressible 2 to regulate the post chemotherapy immune response...
摘要
Abstract
OBJECTIVE:To investigate the efficacy and mechanism of Traditional Chinese Medicine Yishui Shengxue pills(益髓生血丸,YSSX)in mouse models of immunosuppression induced by three chemotherapy drugs. METHODS:We determined an optimal intervention dose of YSSX to investigate efficacy.Changes in immune cell subpopulations were detected by flow cytometry,while immunofluorescence,immunohistochemistry,and other molecular biology methods,were used to verify pathway targets.We used PX-478,an inhibitor of hypoxia-induciblefactor-1α(HIF-1 α),to validate the mechanism of action. RESULTS:Analysis showed that YSSX enhanced the immunity of mouse models of immunosuppression.At the cellular level,YSSX reduced the numbers of myeloid-derived suppressor cells(MDSCs)and enhanced CD8+T cell infiltration.At the molecular level,YSSX reduced the expression levels of hypoxia-inducible factor-1α(HIF-1α),inducible nitric oxide synthase(iNOS),general control nonderepressible 2(GCN2),and eukaryotic initiation factor 2α(eIF2α)in mouse MDSCs,thereby reducing the transcription of HIF-1α,GCN2,and elF2α mRNA.Collectively,these changes led to the increased secretion of interferon-y and interleukin 12,concomitant with a reduction in tumor necrosis factor-α level. CONCLUSIONS:YSSX improved MDSC-mediated immunosuppression in a mouse model after chemotherapy by inhibiting the HIF-1α/iNOS-GCN2/eIF2α signaling axis.关键词
drug therapy/immunosuppression therapy/myeloid-derived suppressor cells/post-chemotherapy/network pharmacology/Yishui Shengxue pillsKey words
drug therapy/immunosuppression therapy/myeloid-derived suppressor cells/post-chemotherapy/network pharmacology/Yishui Shengxue pills引用本文复制引用
QI Yafeng,ZHANG Liying,ZHANG Zhiming,LIU Yu,LIU Yeyuan,LI Yangyang,ZHANG Shangzu,CHEN Yaping,XU Qian,HAO Guoxiong,LIU Yongqi..Therapeutic potential of Traditional Chinese Medicine Yisui Shengxuepills(益髓生血丸)to inhibit hypoxia-inducible factor1alpha and general control nonderepressible 2 to regulate the post chemotherapy immune response...[J].中医杂志（英文版）,2025,45(5):1087-1097,11.基金项目
Supported by the High-end Talents Undertake Provincial Science and Technology Plan Projects(Yangtze River Scholars Award Scheme （）
Gankeji 2021:reference 20-9)and the Gansu Province Integrated Traditional Chinese and Western Medicine Tumor Clinical Medical Research Center(Reference:18JR2FA001) （Reference:18JR2FA001）
