中国中医药信息杂志2025，Vol.32Issue(12)：39-45,7.DOI:10.19879/j.cnki.1005-5304.202504233
基于网络药理学及实验验证探究疏肝止泻方治疗腹泻型肠易激综合征作用机制
Exploration on the Mechanism of Shugan Zhixie Prescription in Irritable Bowel Syndrome with Diarrhea Based on Network Pharmacology and Experimental Validation
摘要
Abstract
Objective To investigate the mechanism of Shugan Zhixie Prescription in treating irritable bowel syndrome with diarrhea(IBS-D)using network pharmacology;To validate the findings through in vivo experiments.Methods Active components and potential targets of Shugan Zhixie Prescription were identified via the TCMSP database.Disease targets for IBS-D were retrieved from GeneCards,DisGeNET and OMIM databases.The intersection of drugs and disease targets was taken,and the protein interaction network was constructed by using STRING database.GO and KEGG pathways were enriched to identify the key signaling pathways of Shugan Zhixie Prescription in the treatment of IBS-D.The rat model of liver depression and spleen deficiency type IBS-D was established by the method of abnormal hunger and satiety,restraint pinch stress and intestinal perfusion of acetic acid.The rats were intervened with low-,medium-and high-dosage of Shugan Zhixie Prescription respectively for 14 days.Serum contents of diamine oxidase(DAO),interleukin(IL)-8,and IL-18 were measured by ELISA.Protein expressions and mRNA expressions of relevant targets in colonic tissue were detected using Western blot and RT-qPCR.Results A total of 26 active components and 553 targets of Shugan Zhixie Prescription were obtained,and 1 930 targets of IBS-D disease were obtained,with 184 drug-disease intersection targets.The possible mechanism was related to NF-κB,AGE-RAGE,Th17 cell differentiation and other signaling pathways.Animal experiments demonstrated that Shugan Zhixie Prescription could significantly reduce defecation frequency,fecal water content,and inflammatory cytokine levels in model rats.It markedly decreased TLR4 and NF-κB protein expressions(P<0.01),while increased AQP3,AQP8 and Occludin protein expressions in colonic tissue(P<0.01,P<0.05).Conclusion Shugan Zhixie Prescription exerts therapeutic effects on IBS-D through multiple pathways and targets,and the mechanism may be related to inhibiting TLR4/NF-κB signaling pathway and promoting intestinal barrier repair.关键词
网络药理学/实验验证/疏肝止泻方/腹泻型肠易激综合征/TLR4/NF-κB通路Key words
network pharmacology/experimental validation/Shugan Zhixie Prescription/irritable bowel syndrome with diarrhea/TLR4/NF-κB signaling pathway分类
医药卫生引用本文复制引用
张金雪,刘俊宏,苏李宁,赖学倩,王丹,陈佳乐,陈雅洁,李红梅,李亚静..基于网络药理学及实验验证探究疏肝止泻方治疗腹泻型肠易激综合征作用机制[J].中国中医药信息杂志,2025,32(12):39-45,7.基金项目
国家中医药管理局青年岐黄学者培养项目(2022年) （2022年）
甘肃省中医药科研课题重点项目(GZKZ-2021-6) （GZKZ-2021-6）
甘肃省重点研发计划(22YFTFA100) （22YFTFA100）
甘肃省科学技术厅创新基地和人才计划(21JR7RA682) （21JR7RA682）
甘肃省省级重点人才项目和陇原青年创新创业人才项目(2022RCXM020) （2022RCXM020）
甘肃省卫生健康行业科研计划项目(GSWSQN2022-01) （GSWSQN2022-01）
