中山大学学报（自然科学版）（中英文）2025，Vol.64Issue(6)：24-30,7.DOI:10.13471/j.cnki.acta.snus.ZR20250107
决明子不同炮制程度的终点量化指标
Quantitative endpoint indicators for different processing degrees of Cassiae Semen
摘要
Abstract
The study aims to investigate the chemical composition transformation patterns of differently processed Cassiae Semen decoction pieces and to establish a scientific evaluation standard for this Lingnan-region herbal medicine.Based on UPLC-Q-TOF-MS2 combined with Feature-Based Molecular Networking(FBMN),the composition differences of raw Cassiae Semen,stir-fried Cassiae Semen and carbonized Cassiae Semen were analyzed,and the HPLC fingerprints of different processing specifications of decoction pieces were constructed.FBMN identified 90 compounds,with 40,42 and 36 compounds(35 shared compounds)were detected in the three types of decoction pieces respectively.The deepening of processing resulted in the breakage of glycosidic bonds of glycosides(e.g.cassiaside B2,rubrofusarin gentiobioside,etc.)and the increase of aglycone content and partial degradation.The discrimination standard was established by the ratio of peak area(RPA)of six transformation-related characteristic peaks:RPA≤0.100 for raw Cassiae Semen;0.200≤RPA≤0.500 for stir-fried Cassiae Semen;RPA>0.500 for carbonized Cassiae Semen.UPLC-Q-TOF-MS2/FBMN could be used to elucidatedynamic transformations patterns of processing,and the RPA index of HPLC fingerprint could provide a scientific basis for objective differentiation of processing specifications and determination of endpoint.关键词
决明子/炮制/化学成分/超高效液相色谱串联四级杆飞行时间质谱/特征分子网络Key words
Cassiae Semen/processing/chemical compositions/UPLC-Q-TOF-MS/feature-based molecular networking分类
医药卫生引用本文复制引用
江玲玲,梁瑞滢,郭子瑜,李哲纯,梁雪,王冰,王淑红,姚新生,苏畅..决明子不同炮制程度的终点量化指标[J].中山大学学报（自然科学版）（中英文）,2025,64(6):24-30,7.基金项目
国家重点研发计划项目(2023YFC3504101) （2023YFC3504101）
国家药品监督管理局中国药品监管科学行动计划重点项目(NMPAJGKX-2023-017) （NMPAJGKX-2023-017）
国家药品监督管理局中药质量研究与评价重点实验室监管科学课题(NMPAKLZ202202) （NMPAKLZ202202）
