重庆医学2025，Vol.54Issue(11)：2515-2521,7.DOI:10.3969/j.issn.1671-8348.2025.11.007
基于16S rDNA基因测序技术探讨糖尿病肾病进展中肠道菌群的变化
Changes in gut microbiota during diabetic nephropathy progression based on 16S rDNA sequencing technology
摘要
Abstract
Objective To investigate changes in gut microbiota during diabetic nephropathy(DN)pro-gression using 16S rDNA sequencing technology.Methods A total of 90 male SD rats were randomly divided into a normal control group(n=10,no modeling,regular feeding)and a model group(diabetes model).The diabetes model was established by a single intraperitoneal injection of streptozotocin(STZ)at 60 mg/kg,with regular feeding.According to the feeding time after modeling,the rats were divided into 2-week,4-week,8-week,and 12-week model groups(fed for 2,4,8,and 12 weeks after model establishment),with 20 rats in each group.Blood urea nitrogen(BUN)was measured using the urease method,serum creatinine(Scr)was deter-mined by the picric acid method,and ELISA was used to detect urinary kidney injury molecule-1(KIM-1)and neutrophil gelatinase-associated lipocalin(NGAL)levels.HE,PAS,and Masson staining were used to observe renal tissue pathological changes.Gut microbiota was collected from the rats,and 16S rDNA gene sequencing was used to analyze the gut microbiota to understand changes in the gut microbiota.Results Compared with the normal control group,the levels of KIM-1 and NGAL in urine of rats in all model groups were significantly increased(P<0.05).Pathological staining results showed that,compared with the normal control group,rats in all model groups exhibited diffuse thickening of the glomerular basement membrane and pathological chan-ges such as local necrosis and vacuolar degeneration in renal tubular epithelial cells.16S rDNA sequencing re-sults indicated that the abundance and structure of intestinal microbiota in rats of all model groups changed.Compared with the normal control group,in the 8-week and 12-week model groups,the relative abundance of Bacteroides and Akkermansia decreased,while the relative abundance of Roseburia,Alloprevotella,Prevotel-laceae-Ga6A1,and Ruminococcaceae UCG-005 increased.Compared with the normal control group,in the 12-week model group,the abundance of Akkermansia decreased and that of Prevotellaceae-NK3B31 increased.Conclusion The abundance and structure of gut microbial community in DN rats under conventional feeding at different time points change significantly,further confirming the"gut-kidney axis"theory.关键词
糖尿病肾病/肠道菌群/16S rDNA测序/菌群丰度/肠肾轴Key words
diabetic nephropathy/gut microbiota/16S rDNA sequencing/microbial abundance/gut-kid-ney axis分类
医药卫生引用本文复制引用
高巧营,张爱民,戈立秀,房杰,陈明慧,贾晓东..基于16S rDNA基因测序技术探讨糖尿病肾病进展中肠道菌群的变化[J].重庆医学,2025,54(11):2515-2521,7.基金项目
天津市卫生健康委员会科技项目(KJ20130) （KJ20130）
天津市中西医结合医院防治关键技术及方案优化2022年度重点专项(NKYY-IIT-2022-009-3). （NKYY-IIT-2022-009-3）
