大豆科学2025，Vol.44Issue(6)：69-77,9.DOI:10.11861/j.issn.1000-9841.2025.06.0069
带状间作模式不同耐荫强度转基因大豆筛选及农艺性状评价
Screening of Transgenic Soybean with Different Shade Tolerance Intensities and Evaluation of Agronomic Traits under Strip Intercropping Systems
摘要
Abstract
In order to analyze the shade tolerance characteristics of transgenic soybean lines under different corn-soybean strip intercropping systems and screen transgenic soybean lines suitable for strip intercropping patterns,we studied 10 agronomic traits of 17 transgenic soybean lines with large trial planting areas and good yield performance.We set up two strip intercropping patterns with a field corn and soybean planting ratio of 2∶2 and 4∶4.The shade tolerance-related agronomic traits of different transgenic soybean lines were measured at maturity.The shade tolerance of different transgenic soybean lines was evaluated by multivariate analysis such as principal component,cluster and stepwise regression.The results showed that shade stress had the greatest effect on the number of soybean branches,with a coefficient of variation of 73.88%;it had a smaller effect on the number of nodes,balance point and plant height,with coefficients of variation of 10.91%,10.50%and 9.98%respectively.Through single-trait correlation analysis,it was found that the bottom pod height was significantly negatively correlated with the number of pods,and significantly negatively correlated with the stem weight and pod weight;the number of nodes was significantly positively correlated with plant height.The principal component analysis extracted 4 principal components,with a cumulative contribution rate of 80.42%.The membership function method was used to calculate the comprehensive shade tolerance evaluation value(D)of each transgenic soybean line at maturity.According to the D value,the 17 soybean lines were divided into 3 categories using the system cluster analysis method,namely strong shade tolerance type,including 5 soybean lines;medium shade tolerance type,including 3 soybean lines;and weak shade tolerance type,including 9 soybean lines.Zhongliandou 3051 had the strongest shade tolerance,while Zhongliandou 1343 had the weakest.The soybean lines with strong shade tolerance were Zhongliandou 3051,Zhongliandou 3048,Zhongliandou 3047,Zhongliandou 1349 and Zhongliandou 3050.The stepwise regression analysis of the D value showed that the shade tolerance evaluation model was D=－0.541 －1.601X1+0.882X9+1.423X5+0.674X10+0.368X4,and through this model,it was found that bottom pod height,pod weight,number of nodes,yield and stem diameter can be used as evaluation indicators for soybean shade tolerance in the corn-soybean strip intercropping systems.关键词
带状间作/耐荫强度/转基因大豆品系/主成分分析/隶属函数法Key words
strip intercropping/shade tolerance characteristics/transgenic soybean lines/principal component analysis/membership function method引用本文复制引用
杨晓芳,庞佳莹,孟凡钢,饶德民,于德彬,王弼琨,王晓慧,张伟..带状间作模式不同耐荫强度转基因大豆筛选及农艺性状评价[J].大豆科学,2025,44(6):69-77,9.基金项目
吉林省自然科学基金面上项目(20240101241JC). （20240101241JC）
