渔业研究2025，Vol.47Issue(6)：748-757,10.DOI:10.14012/j.jfr.2025089
红尾皇冠鱼源彭氏变形杆菌的分离、鉴定及药敏实验
Isolation,identification and antimicrobial susceptibility testing of Proteus penneri from Aequidens rivulatus
摘要
Abstract
[Objective]This study aimed to conduct isolation,identification,and antimicrobial susceptibility testing of pathogenic bacteria from diseased red-tailed crown fish(Aequidens rivulatus),providing theoretical basis and practical guidance for the prevention and control of diseases in A.rivulatus.[Methods]The strain was identified through morphological observation,physiological and biochemical characteristic analysis,16S rRNA gene sequencing,and phylogenetic tree construction.Its susceptibility to antimicrobial agents was also determined.[Results]The results showed that the physiological and biochemical characteristics of the isolated bacterium were consistent with those of Proteus penneri.Meanwhile,molecular identification results and phylo-genetic tree analysis indicated that the strain had a close genetic relationship with P.penneri,thus confirming the isolated pathogen as P.penneri.In the regression infection test,the isolated strain was found to be pathogen-ic to A.rivulatus.For individual A.rivulatus weighing approximately 50 g,the median lethal dose(LD50)was 5.16×105 CFU/ind.Antimicrobial susceptibility testing showed that the strain was sensitive to ciprofloxacin,en-rofloxacin,and trimethoprim-sulfamethoxazole,but resistant to penicillin and ceftriaxone.[Conclusion]In summary,the study confirms that P.penneri is the pathogenic bacterium causing diseases in A.rivulatus.On the basis of ensuring the safety of aquatic drug use,enrofloxacin and ciprofloxacin can be used as effective drugs for treating infections in A.rivulatus.关键词
红尾皇冠鱼/彭氏变形杆菌/细菌分离与鉴定/致病性/药敏实验Key words
Aequidens rivulatus/Proteus penneri/bacterial isolation and identification/pathogenicity/antimicrobial susceptibility test分类
农业科技引用本文复制引用
金楠霖,盛冬,郝可心,张雨,任旭,杨家豪,叶仕根..红尾皇冠鱼源彭氏变形杆菌的分离、鉴定及药敏实验[J].渔业研究,2025,47(6):748-757,10.基金项目
辽宁省科技计划(2024JH2/102600072) （2024JH2/102600072）
