渔业研究2025，Vol.47Issue(6)：770-780,11.DOI:10.14012/j.jfr.2025055
基于适配体的纳米金比色法快速检测河豚毒素
Rapid detection of tetrodotoxin by aptamer-based gold nanoparticles colorimetric method
摘要
Abstract
[Background]Tetrodotoxin(TTX),a potent neurotoxin widely distributed in marine organisms such as pufferfish,poses a significant public health threat caused by accidental consumption of TTX-contamin-ated aquatic products.This toxin can induce respiratory failure and fatal outcomes,underscoring the urgent need for highly efficient and sensitive detection technologies to ensure food safety.[Objective]This study aims to construct a simple,visual colorimetric detection method based on aptamer-modified gold nanoparticles(AuNPs)sensor.[Methods]AuNPs were prepared by the sodium citrate reduction method,and characterized by trans-mission electron microscopy(TEM)and ultraviolet-visible(UV-Vis)absorption spectroscopy.Aptamer(Apt)were adsorbed onto the surface of AuNPs through electrostatic interaction,which protected AuNPs from ag-gregation and color change in high-salt environments to a certain extent.When TTX existed in the system,it specifically bound to Apt,causing AuNPs to aggregate and change color.After optimizing the experimental conditions,the ratio of A620/A521 at different TTX concentrations was determined by UV spectroscopy to estab-lish a linear regression equation,calculate the limit of detection,and perform specificity analysis and spike-re-covery experiments.[Results]The prepared AuNPs had uniform particle size(approximately 15 nm)and a maximum absorption peak at 521 nm.Based on the result,a visual TTX detection method using an Apt-AuNPs colorimetric sensor was constructed.The optimal experimental parameters determined through condition optim-ization were as follows:AuNPs volume fraction of 45%,NaCl concentration of 300 mmol/L,NaCl reaction time of 10 min,Apt concentration of 80 nmol/L,Apt incubation time of 25 min,TTX-Apt incubation time of 20 min,TTX-Apt incubation temperature of 10 ℃,and TTX-Apt pH of 5.Under the optimized conditions,TTX detec-tion showed a good linear relationship between TTX concentration(19.53～312.5 ng/mL)and the corresponding A620/A521 value,with a linear correlation coefficient(R2)of 0.995 7 and a limit of detection of 12.97 ng/mL.In the spike recovery experiments,the recovery rates of TTX ranged from 104.2%to 108.4%,with relative stand-ard deviations(RSD)of 0.85%～1.77%.[Conclusion]The study developes an aptamer-based AuNPs colorimet-ric method for TTX detection,which is expected to provide a simple,rapid,visual,and accurate approach for TTX analysis.关键词
河豚毒素(TTX)/适配体(Apt)/金纳米颗粒(AuNPs)/比色法Key words
tetrodotoxin(TTX)/aptamer(Apt)/gold nanoparticles(AuNPs)/colorimetry分类
农业科技引用本文复制引用
刘子竞,蔡水淋,黄文树,陈小红,乔琨,许旻,刘智禹..基于适配体的纳米金比色法快速检测河豚毒素[J].渔业研究,2025,47(6):770-780,11.基金项目
福建省自然科学基金面上项目(2023J011323) （2023J011323）
福建省海洋保护与发展专项(HYBF20200066-8) （HYBF20200066-8）
福建省海洋服务与渔业高质量发展专项资金项目(FJHY-YYKJ-2024-1-1) （FJHY-YYKJ-2024-1-1）
