辽宁石油化工大学学报2025，Vol.45Issue(6)：11-18,8.DOI:10.12422/j.issn.1672-6952.2025.06.002
基于响应面法的石蜡降解菌群培养条件优化
Optimization of Culture Conditions for Paraffin Degrading Bacterial Communities Based on Response Surface Methodology
摘要
Abstract
To enhance the flowability of waxy crude oil,seven paraffin-degrading strains were screened using paraffin as the sole carbon source.Their paraffin removal efficiency,emulsification capability,and hydrophobicity were evaluated to identify high-efficiency degraders.Strains with optimal growth characteristics were selected based on growth curves,and microbial consortia were constructed through optimized combinations.Cultivation conditions were further refined using single-factor experiments and response surface methodology(RSM).The orthogonal experimental results indicate that when constructing the compound microbial consortium with Broussonetia papyrifera,the inoculation amounts(volume fractions)of strains H1,H3,and H4 should be 1.0%,2.0%,and 0.5%respectively,respectively,the paraffin removal rate of the consortium reached 58.7%.The optimized cultivation conditions were determined as follows:temperature at 41.8℃,inoculation volume at 3.0%,and shaking speed at 181 r/min.The influence of factors on paraffin removal followed the order:cultivation temperature>inoculation volume>shaking speed.After optimization,the paraffin removal rate of the consortium increased to 62.1%.When the microbial consortium was applied to treat waxy crude oil,the viscosity reduction rate reached 51.5%,significantly improving the fluidity of the crude oil and thereby enhancing pipeline transportation efficiency.关键词
含蜡原油/正交实验/石蜡降解菌菌群/响应面优化Key words
Waxy crude oil/Orthogonal experiment/Paraffin degrading bacterial community/Response surface optimization分类
能源科技引用本文复制引用
杨立鉴,王慧,万海,庞浩..基于响应面法的石蜡降解菌群培养条件优化[J].辽宁石油化工大学学报,2025,45(6):11-18,8.基金项目
辽宁省教育厅面上项目(JYTMS20231438) （JYTMS20231438）
辽宁省联合基金资助项目(U1908203). （U1908203）
