分析测试学报2025，Vol.44Issue(12)：2449-2461,13.DOI:10.12452/j.fxcsxb.250430334
指纹图谱定性分析与多成分定量分析相结合的3种金荞麦制剂质量比较
Quality Comparison of Three Fagopyri Dibotryis Rhizoma Preparations by Combining Fingerprint Qualitative Analysis with Multi-component Quantitative Analysis
摘要
Abstract
This study conducted a comparative quality evaluation of three Fagopyri Dibotryis Rhizoma(FDR)preparations named FDR Tablets(FDRT),FDR Capsules(FDRC),and Weimaining Cap-sules(WMNC)by combining fingerprint qualitative analysis with multi-component quantitative analy-sis.Fingerprints of 20 batches of FDRT,11 batches of FDRC,and 15 batches of WMNC were deter-mined by high performance liquid chromatography,40 common peaks in the fingerprints were demar-cated,and 67 components were identified in these 40 common peaks by ultra-fast liquid chromatogra-phy-quadrupole time-of-flight mass spectrometry(UFLC-Q-TOF-MS/MS).Among these,6 tannins including procyanidin B1(PB1),procyanidin B3(PB3),procyanidin C2(PC2),procyanidin B2(PB2),procyanidin C1(PC1),and procyanidin A2(PA2),3 phenolics including gallic acid,pro-tocatechuic acid,and protocatechualdehyde,and 4 flavonoids including catechin,epicatechin,epicatechin gallate(ECG),and rutin were confirmed by reference substance.Hierarchical cluster analysis,principal component analysis,and orthogonal partial least squares discriminant analysis were conducted by using the quantified peak areas of the common peaks as variables.The results con-sistently showed significant differences in quality between FDRT and FDRC compared to WMNC,and there were also some differences in quality between FDRT and FDRC themselves.Quantitative determination was conducted on a total of 10 components,which were verified by comparison with reference substance and exhibited good separation.The results revealed that,with the exception of no significant difference in the mean content of catechin between FDRC and WMNC,as well as in PA2 between FDRT and FDRC(P>0.05),the mean content of all 10 components across the three FDR preparations showed significant differences(P<0.01 or P<0.05).Specifically:gallic acid,protocatechuic acid,and protocatechualdehyde exhibited FDRC>FDRT>WMNC,PB1 and PB3 showed FDRC>WMNC>FDRT,epicatechin,PC1,and ECG demonstrated WMNC>FDRC>FDRT,catechin displayed both FDRC and WMNC>FDRT,and PA2 exhibited WMNC>both FDRC and FDRT.In summary,the main components contained in the three FDR preparations are basically the same;however,there are differences in quality,with FDRT and FDRC demonstrating notable dif-ferences compared to WMNC.关键词
金荞麦片/金荞麦胶囊/威麦宁胶囊/质量比较/指纹图谱/超快速液相色谱-四极杆飞行时间质谱/模式识别/多成分定量分析Key words
Fagopyri Dibotryis Rhizoma Tablets/Fagopyri Dibotryis Rhizoma Capsules/Weimain-ing Capsules/quality comparison/fingerprint/UFLC-Q-TOF-MS/MS/pattern recognition/multi-component quantitative analysis分类
化学化工引用本文复制引用
何梦,李余佳,曹溢,戴莹,秦楠,窦志华..指纹图谱定性分析与多成分定量分析相结合的3种金荞麦制剂质量比较[J].分析测试学报,2025,44(12):2449-2461,13.基金项目
南通市科技计划重点项目新冠肺炎专项(MS12022012) （MS12022012）
