分析测试学报2025，Vol.44Issue(12)：2497-2506,10.DOI:10.12452/j.fxcsxb.250403258
基于UPLC-Q-TOF-MS/MS技术结合多元统计分析方法研究大承气汤复方单煎与合煎差异成分
Investigation on Differential Components Between Single Decoction and Combined Decoction Dachengqi Decoction Using UPLC-Q-TOF-MS/MS Technology Combined with Multivariate Statistical Analysis
摘要
Abstract
By using ultra performance liquid chromatography-quadrupole-time of flight high resolu-tion mass spectrometry(UPLC-Q-TOF-MS/MS)technology,a high-resolution mass spectrometry da-tabase of the chemical components of Dachengqi Decoction was constructed.Combined with multivar-iate statistical analysis,the differences in the chemical components of the mixed freeze-dried powder of Dachengqi Decoction prepared by simultaneous decoction and single decoction were studied.The results showed that the main chemical components of the mixed freeze-dried powder of Dachengqi De-coction prepared by simultaneous decoction and single decoction were the same,but the contents were different.There were 18 components with significant differences in content,mainly including 3 flavonoid glycosides,3 anthraquinones,2 organic acids,3 lignans,4 phenylethanol glycosides,1 phenol and 2 triterpenoids.Simultaneous decoction could significantly increase the contents of rutin,naringenin,hesperetin,limonin and verbascoside in Dachengqi Decoction,and decrease the con-tents of rhein and rhein-8-O-β-D-glucoside.These components are the key components for the syner-gistic effect and reduced toxicity of Dachengqi Decoction after compatibility,which to some extent ex-plains the scientific connotation of the reduced toxicity and enhanced efficacy of Dachengqi Decoction after compatibility.The research results can provide a reference for the rational application and evalu-ation of Dachengqi Decoction compound preparations and formula granules in clinical practice.关键词
大承气汤/超高效液相色谱-四极杆-飞行时间高分辨质谱/单煎/合煎/化学一致性Key words
Dachengqi Decoction/ultra performance liquid chromatography-quadrupole-time of flight high resolution mass spectrometry/single decoction/combined decoction/chemical consis-tency分类
化学化工引用本文复制引用
陈婉纯,毕晓黎,刘权,刘焰嫦,卢文森,肖观林..基于UPLC-Q-TOF-MS/MS技术结合多元统计分析方法研究大承气汤复方单煎与合煎差异成分[J].分析测试学报,2025,44(12):2497-2506,10.基金项目
广州地区中医药重大科技项目(2025QN003) （2025QN003）
广东省中医药局科研项目(20251034) （20251034）
广东省基础与应用基础研究基金项目(2024A1515012809) （2024A1515012809）
广州中医药大学校院联合科技创新基金(GZYSE2024G07) （GZYSE2024G07）
