分析测试学报2025，Vol.44Issue(12)：2516-2525,10.DOI:10.12452/j.fxcsxb.250413288
改良QuEChERS结合超高效液相色谱-串联质谱法同时测定消毒产品中20种违禁添加药物
Simultaneous Determination of 20 Illegally Added Drugs in Disinfection Products by Modified QuEChERS/Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
An analytical method using ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was established for the simultaneous determination of 20 illegally add-ed drugs in disinfection products.Three dosage forms of samples were dispersed by water induction,extracted with acidified methanol,and purified with N-propyl ethylenediamine(PSA)adsorbent.The 20 target analytes were separated on a Waters AcquityTM UPLC BEH C18 column(100 mm×2.1 mm,1.7 μm)with 0.05%formic acid water and 0.05%formic acid acetonitrile solution as mobile phase.The data were acquired using an electrospray ionization(ESI)source with positive/negative switching mode under multiple reaction monitoring(MRM),and quantified via the isotope internal standards.The 20 analytes exhibited good linearity in their respective mass concentration ranges,with correla-tion coefficients(r²)of 0.999 4-0.999 9.The method detection limits(LODs)ranged from 0.5 μg/kg to 10.0 μg/kg,and the method quantification limits(LOQs)were 1.25-25.0 μg/kg.The spiked re-covery tests were conducted at low,medium and high levels,showing average recoveries of 84.5%-109%and relative standard deviations(RSDs,n=6)of 0.22%-8.4%.The established analytical method was applied to the determination of actual samples,and 8 samples were detected with illegal drugs.With simplified pretreatment,high purification efficiency,and short analytical runtime,the devel-oped method achieves superior sensitivity and selectivity.It is capable of simultaneously meeting the requirements for both qualitative screening and quantitative analysis.It can be used for rapid analysis of various illegally added drugs in a large number of disinfection products.关键词
消毒产品/违禁添加药物/超高效液相色谱-串联质谱/同位素内标Key words
disinfection products/illegally added drugs/UPLC-MS/MS/isotope internal standard分类
化学化工引用本文复制引用
赵紫微,詹子悦,戴雁羽,谢继安,丁刚,徐庆华,刘柏林..改良QuEChERS结合超高效液相色谱-串联质谱法同时测定消毒产品中20种违禁添加药物[J].分析测试学报,2025,44(12):2516-2525,10.基金项目
2024年中国疾控中心标准化前期研究项目(BZ2024-Q009) （BZ2024-Q009）
2024年度安徽省卫生健康科研一般项目(AHWJ2024Aa20499) （AHWJ2024Aa20499）
