高等学校化学学报2025，Vol.46Issue(12)：24-36,13.DOI:10.7503/cjcu20250204
基于构象自适荧光团构建的比率型荧光探针及脂滴原位可视化研究
Fluorescent Probes Constructed Based on Conformationally-adaptive Fluorophores for the In-situ Visualization of Lipid Droplets
摘要
Abstract
In this study,a ratiometric fluorescent probe,TPP-DPAC,was developed for dual labeling of lipid droplets(LDs)and mitochondria.This probe was constructed by conjugating the lipophilic"conformational adaptive"fluorophore N,N'-diphenyl-dihydrodibenzo[a,c]phenazine(DPAC)—which exhibits dual fluorescence emission—with the mitochondrial-targeting group triphenylphosphine(TPP).By co-incubating OA-induced HepG2 cells with the probe to stimulate LD formation,it was demonstrated that TPP-DPAC could simultaneously label both LDs and mitochondria.The fluorescence imaging co-localization coefficients(Rr)were 0.96 and 0.95,respectively.Further experiments revealed that the probe undergoes dynamic changes in red/blue fluorescence signals due to its conformational adaptation to the local cellular microenvironment,thereby enabling in situ tracing of dynamic cellular processes such as LD formation and fusion.This ratiometric fluorescent probe provides a visualization tool for monitoring intracellular LD metabolism and its interaction with mitochondria,and offers a new strategy for studying interactions between LDs and other subcellular organelles.关键词
荧光探针/脂滴/线粒体/荧光成像Key words
Fluorescent probe/Lipid droplet/Mitochondria/Fluorescence imaging分类
化学化工引用本文复制引用
周意超,田贺,韩海浩,王辰瀚,胡习乐,贺晓鹏..基于构象自适荧光团构建的比率型荧光探针及脂滴原位可视化研究[J].高等学校化学学报,2025,46(12):24-36,13.基金项目
国家自然科学基金(批准号:92253306,82130099,22477030)资助. Supported by the National Nature Science Foundation of China(Nos.92253306,82130099,22477030). （批准号:92253306,82130099,22477030）
