西部中医药2025，Vol.38Issue(11)：1-7,7.DOI:10.12174/j.issn.2096-9600.2025.11.01
溃结宁膏穴位敷贴对溃疡性结肠炎大鼠氧化应激损伤的保护作用研究
Study on the Protective Effects of Kuijiening Ointment for Acupoint Application on Oxidative Stress Injury in Ulcerative Colitis Rats
摘要
Abstract
Objective:To discuss the protective effects of Kuijiening ointment for acupoint application on oxidative stress injury in ulcerative colitis(UC)rats in"deficiency"and"stagnation"state,and its potential mechanism based on Nrf2/HO-1/Mfn1 and Mfn2.Methods:All 51 SPF-grade male SD rats were randomized into normal control group(10 rats)and the modeling group(41 rats).The modeling group were prepared into UC rat models in"deficiency"and"stagnation"state by intragastric administration of adenine and iced senna leaves in stages and TNBS/ethanol method.After successfully modeling,the modeling group were randomly allocated to the model group,non acupoint application group,acupoint application group and sulfasalazine group,and they received different intervention methods.Normal control group and the model group were given medicine-free cataplasm matrix acupoint application,topical application of Kuijiening ointment at Zusanli(ST36),Tianshu(ST25),Shenshu(BL23)and Geshu(BL17)was administered to acupoint application group;topical application of Kuijiening ointment at well-muscled parts of the buttocks in non acupoint application group;sulfasalazine group received medicine-free cataplasm matrix acupoint application and gastric lavage of sulfasalazine solution,and the remaining groups were drenched with the equivalent amount of physiological saline,once each day,intervened for 28 days consecutively.To observe the mortality and macroscopic representation of the rats in different groups,to assess ocular trauma score(OTS)and pathological injury scores of colon tissue,fluorescent quantitative polymerase chain reaction(FQ-PCR)was adopted to measure the expressions of Keap1,Nrf2,Mfn1 and Mfn2 mRNA in colon tissue of the rats;WB was utilized to detect the levels of Keap1,Nrf2 and HO-1 protein in the colon tissue of the rats.Results:Compared with normal control group,OTS and pathological injury scores of colon tissue were elevated in the model group(P<0.01),Keap1 protein and mRNA expressions increased(P<0.01),the levels of Nrf2 protein and mRNA,Mfn1 mRNA,Mfn2 mRNA and HO-1 protein remarkably reduced(P<0.05);compared with the model group,OTS and pathological injury scores of colon tissue decreased in acupoint application and sulfasalazine group(P<0.01),Keap1 protein and mRNA expressions down regulated(P<0.01),the expressions of Nrf2 protein and mRNA,Mfn1 mRNA,Mfn2 mRNA and HO-1 protein were raised(P<0.05),the differences had no statistical meaning in the changes of the above indexes in non acupoint application group;when acupoint application group was compared with non acupoint application group,OTS and pathological injury scores of colon tissue were lowered(P<0.01),the levels of Keap1 protein and mRNA were cut(P<0.01),the expressions of Nrf2 protein and mRNA,Mfn1 mRNA,Mfn2 mRNA and HO-1 protein up regulated(P<0.05);the difference showed no statistical meaning in the indexes between acupoint application group and sulfasalazine group.Conclusion:Kuijiening ointment for acupoint application exert the protective effects against oxidative stress injury in UC rats by activating Nrf2/HO-1 signaling pathway,upregulating the levels of Mfn1 and Mfn2 mRNA and improving mitochondrial damage.关键词
结肠炎,溃疡性/溃结宁膏/穴位敷贴/信号通路/线粒体融合蛋白1/线粒体融合蛋白2/"虚、瘀"状态Key words
colitis,ulcerative/Kuijiening ointment/acupoint application/signaling pathway/mfn1/mfn2分类
医药卫生引用本文复制引用
吴珊,朱莹,张建伟,王生华,张娜,王燚霈..溃结宁膏穴位敷贴对溃疡性结肠炎大鼠氧化应激损伤的保护作用研究[J].西部中医药,2025,38(11):1-7,7.基金项目
国家自然科学基金(81874466) （81874466）
中医内科重大疾病防治研究与转化教育部重点实验室开放基金(ZYNK201705) （ZYNK201705）
湖南省中医药科研计划项目(2020043) （2020043）
湖南省教育厅科学研究项目(18C0401). （18C0401）
