西部中医药2025，Vol.38Issue(11)：8-11,4.DOI:10.12174/j.issn.2096-9600.2025.11.02
葛根素对肝癌小鼠肝细胞氧化应激损伤的干预机制研究
Study on Intervention Mechanism of Puerarin on Oxidative Stress Injury in Hepatocytes of Liver Cancer Mice
摘要
Abstract
Objective:To explore the intervention effects of puerarin on oxidative stress injury in hepatocytes of liver cancer mice,and its mechanism of regulating the expressions of BMP-9 and VEGF-165.Methods:A total of 36 mice were allocated to blank group,model group and experiment group with 12 in each according to random number method.After one week of adaptive feeding,the mice in the model group and the experiment group were established into liver cancer models by hypodermic injection of human hepatocellular carcinoma HuH-7 cell suspension into left armpit,and the blank group was unhandled.After successfully modeling,the blank group and the model group received peritoneal injection of physiological saline each day,and puerarin injection was given to the experiment group every day.After four consecutive weeks of the intervention,to detect the expressions of ROS,SOD,MDA and GSH in the mice of different groups;HE was used to observe pathological changes of liver tissue in the mice of different groups;RT-PCR and WB were applied to measure the levels of BMP-9,VEGF-165 mRNA and protein in different groups.Results:Compared with the blank group,liver tissue cells were severely damaged in the model group,an increase in ROS and MDA,and a decrease in SOD and GSH were found(all P<0.05);the expressions of BMP-9,VEGF-165 mRNA and protein were elevated in liver tissue(P<0.05).Compared with the model group,the changes including significant improvement of liver tissue cell morphology,the decrease in the levels of serum ROS and MDA,and the increase in the levels of SOD and GSH were seen in the experiment group(P<0.05),oxidative stress injury of hepatic cells was relieved obviously;the expressions of BMP-9,VEGF-165 mRNA and protein were reduced in liver tissue(P<0.05).Conclusion:Puerarin could improve oxidative stress injury in liver cancer mice,and it might be related to the regulation of BMP-9 and VEGF-165 expression by puerarin.关键词
肝癌/葛根素/氧化应激损伤/小鼠/实验Key words
liver cancer/puerarin/oxidative stress injury/mice/experiment分类
医药卫生引用本文复制引用
李琳婵,鱼麦侠,王松海..葛根素对肝癌小鼠肝细胞氧化应激损伤的干预机制研究[J].西部中医药,2025,38(11):8-11,4.基金项目
陕西省重点研发计划项目(2020SF-348) （2020SF-348）
陕西省中医药管理局中医药科研课题(2019-LC014). （2019-LC014）
