西部中医药2025，Vol.38Issue(11)：47-60,14.DOI:10.12174/j.issn.2096-9600.2025.11.09
生、熟地黄"生熟异用"功效差异的机制及质量标志物探析
Preliminary Study on the Mechanism of Efficacy Difference between Raw and Prepared Rhizoma Rehmanniae,and Quality Markers
摘要
Abstract
Objective:To explore scientific connotation of efficacy difference between raw and prepared rhizoma rehmanniae,and potential quality markers.Methods:Network pharmacology was adopted to construct the network of"differential ingredients-targets-signaling pathways"for raw and processed rhizoma rehmanniae before and after processing.Differential components were screened out through literature retrieval,the potential targets were identified using Swiss Target Prediction;STRING database was applied to screen out the targets with highest confidence,Cytoscape 3.7.2 was adopted to construct protein-protein interaction network,and the key targets were screened in light of Degree values;Metascape data base was applied to conduct GO and KEGG signaling pathway enrichment analysis of the core targets;Autodock Vina platform was adopted to perform molecular docking,for the identification of"core ingredients-core targets-core pathways"that mediates pharmacological differences before and after processing,and potential efficacy-related quality markers;the differential components were analyzed based on"five-principle"of Q-marker for Traditional Chinese Medicine,and the key Q-markers of efficacy difference were predicted before and after processing.Results:Literature retrieval identified 17 differential ingredients,and yielded 299 potential targets after prediction,96 targets were obtained after overlapping(containing 66 key targets);enrichment analysis yielded 4,139 GO annotations and 334 KEGG pathways,consequently to screen out one core pathway(neuroactive ligand-receptor interactions),one core target(MMP12)and four core polysaccharides including raffinose,levidulinose,saccharose and stachyose,and the above core elements jointly participated in the mediation of efficacy difference of raw and processed rhizoma rehmanniae;meanwhile,rehmanniae polysaccharides were the potential efficacy difference Q-markers.In light of Q-marker theory,rehmannioside D,verbascoside,leonuride,catalpol,rehmannia glutinosa polysaccharide,5-hydroxyme-thylfurfural and isoverbascoside were the potential Q-markers of efficacy difference before and after processing.Conclusion:Raw and prepared rhizoma rehmanniae could regulate efficacy difference via signaling pathway of"neuroactive ligand-receptor interactions",MMP12 core targets and four core polysaccharides including raffinose,levidulinose,saccharose and stachyose;rehmannioside D,verbascoside,leonuride,catalpol,rehmannia glutinosa polysaccharide,5-hydroxymethylfurfural and isoverbascoside could be taken as the quality markers to mediate efficacy difference between the raw and processed ones.关键词
生地黄/熟地黄/网络药理学/生熟异用/功效差异/质量标志物Key words
raw rhizoma rehmanniae/prepared rhizoma rehmanniae/network pharmacology/different uses of raw and processed herbs/efficacy difference/quality markers分类
医药卫生引用本文复制引用
杨鹤年,张津铖,吴宿慧,李寒冰,李根林..生、熟地黄"生熟异用"功效差异的机制及质量标志物探析[J].西部中医药,2025,38(11):47-60,14.基金项目
国家自然科学基金(81503363) （81503363）
河南省科技厅科技研发专项资助项目(192102310173). （192102310173）
