医学分子生物学杂志2025，Vol.22Issue(6)：539-547,9.DOI:10.3870/j.issn.1672-8009.2025.06.002
乳糜泻中lncRNA GAS5通过靶向miR-155调节Th17/Treg失衡
LncRNA GAS5 Regulates Th17/Treg Imbalance in Celiac Disease by Targeting miR-155
摘要
Abstract
Objective To investigate how long non-coding RNA(lncRNA)GAS5 regulates Th17/Treg imbalance through targeting miR-155 in celiac disease(CeD).Methods Peripheral blood was collected from 20 CeD patients(CeD group)and 20 healthy individuals without CeD or other autoimmune diseases(healthy group),CD4+T cells were isolated from both groups.the ex-pression levels of lncRNA GAS5 and miR-155 in CD4+T cells from CeD patients and the control group were dected by qRT-PCR.Flow cytometry was used to analyze the Th17/Treg ratio.Pearson statistical analysis was performed to evaluate the correlation between the expression levels of lncRNA GAS5 and miR-155 with the Th17/Treg ratio in CeD patients.In vitro,CD4+T cells from the CeD patients were divided into 7 groups:control group,pcDNA3.1-NC group,pcDNA3.1-GAS5 group,miR-NC group,miR-155-mimics group,pcDNA3.1-GAS5+miR-NC group,and pcD-NA3.1-GAS5+miR-155-mimics group.qRT-PCR was used to detect the expression levels of ln-cRNA GAS5 and miR-155 in each group.Edu staining and Hoest33342 staining was used to detect the proliferation and apoptosis of CD4+T cells,respectively.Immunofluorescence staining was used to measure the expression of IL-17 and Foxp3.Flow cytometry was used to detect the proportion of IL-17+and Foxp3+cells in each group.Results Compared to those in the healthy group,the ex-pression level of lncRNA GAS5 in the CeD group was decreased,while the expression level of miR-155 was increased.lncRNA GAS5 was negatively correlated with the Th17/Treg ratio(r=-0.65,P＜0.05),and miR-155 was positively correlated with the Th17/Treg ratio(r=0.70,P＜0.05).In CD4+T cells from the CeD group,the pcDNA3.1-GAS5 group showed increased ex-pression levels of lncRNA GAS5 and Foxp3,increased proliferation rate of CD4+T cells,and in-creased proportion of Foxp3+cells(allP＜0.05),while the expression levels of miR-155 and IL-17,the apoptosis rate of CD4+T cells,and the proportion of IL-17+cells were decreased(allP＜0.05)when compared with those in the pcDNA3.1-NC group.When compared with the miR-NC group,the miR-155-mimics group showed decreased Foxp3 expression level,decreased prolifera-tion rate of CD4+T cells,and decreased proportion of Foxp3+cells(allP＜0.05),while the ex-pression levels of miR-155 and IL-17,the apoptosis rate of CD4+T cells,and the proportion of IL-17+cells were increased(allP＜0.05).Compared with the pcDNA3.1-GAS5+miR-NC group,the pcDNA3.1-GAS5+miR-155-mimics group showed decreased Foxp3 expression level,decreased proliferation rate of CD4+T cells,and decreased proportion of Foxp3+cells(allP＜0.05),while the expression levels of miR-155 and IL-17,the apoptosis rate of CD4+T cells,and the proportion of IL-17+cells were increased(allP＜0.05).Conclusion lncRNA GAS5 regulates Th17/Treg im-balance by targeting miR-155,affecting the proliferation and apoptosis of CD4+T cells in CeD pa-tients,thereby influencing IL-17 and Foxp3 expression.This suggests that lncRNA GAS5 may serve as a potential therapeutic target for CeD.关键词
乳糜泻/长链非编码RNA GAS5/微小RNA-155/辅助性T细胞17/调节性T细胞Key words
celiac disease/long non-coding RNA GAS5/microRNA-155/T help type 17 cells/regulatory T cells分类
医药卫生引用本文复制引用
冯燕,李婷,王曼,刘卫东,王春,高峰..乳糜泻中lncRNA GAS5通过靶向miR-155调节Th17/Treg失衡[J].医学分子生物学杂志,2025,22(6):539-547,9.基金项目
新疆维吾尔自治区自然科学基金(No.2023D01C76) This work was supported by a grant from the Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2023D01C76) （No.2023D01C76）
