医学分子生物学杂志2025，Vol.22Issue(6)：557-562,6.DOI:10.3870/j.issn.1672-8009.2025.06.004
miR-214-5p通过FANCA调节神经母细胞瘤DNA损伤和化疗敏感性
Effect of miR-214-5p on DNA Damage and Chemotherapy Sensitivity in Neuroblastoma Cells by Regulation of FANCA
摘要
Abstract
Objective To explore the effect and mechanism of microRNA-214-5p(miR-214-5p)on DNA damage and chemotherapy sensitivity in neuroblastoma(NB).Methods The rela-tionship between Fanconi anemia complementary group A(FACNA)protein and clinicopathological characteristics of NB was analyzed by TARGET database.NB cell lines with low expression of miR-214-5p and overexpression/low expression of FANCA were constructed,and their effects on the pro-liferation of NB in vivo and in vitro were detected by cell counting kit-8(CCK-8)and transplanted tumor model.The targeted relationship between miR-214-5p and FANCA was verified.Results FANCA was screened out as the intersection gene correlated with NB in GEO and TARGET databas-es,and its expression level was related to cells proliferation pathways,and the prognosis and clini-copathological characteristics of NB.Compared with those in the empty vector(Vector)group,the cells viability,and the volume and weight of transplanted tumors were increased in the FANCA group(P＜0.05).Compared with those in the shNC group,the cells viability was decreased in the shFANCA1/2 groups(P＜0.05).Compared with those in the control group,the phosphorylated histone γ-H2AX was increased after treated with 1 mmol/L H2O2(P＜0.05),but γ-H2AX was decreased after FANCA overexpression(P＜0.05).With the increase of doxorubicin concentration,cells viability was decreased,but it was higher in the FANCA group than in the Vector group(P＜0.05).There was a targeted relationship between miR-214-5p and FANCA.Conclusion miR-214-5p can inhibit the proliferation of NB cells by reducing FANCA expression level,and regulate the DNA damage and chemotherapy sensitivity in cells.关键词
神经母细胞瘤/微小RNA-214-5p/范可尼贫血互补组A型蛋白/DNA损伤/化疗敏感性Key words
neuroblastoma/microRNA-214-5p/fanconi anemia complementary group A pro-tein/DNA damage/chemotherapy sensitivity分类
医药卫生引用本文复制引用
赵聪慧,刘冰,刘佳佳,崔凌,刘晓霞,雷建华..miR-214-5p通过FANCA调节神经母细胞瘤DNA损伤和化疗敏感性[J].医学分子生物学杂志,2025,22(6):557-562,6.基金项目
河北省医学科学研究课题(No.20241660) This work was supported by a grant from the Medical Science Research Project of Hebei Province(No.20241660) （No.20241660）
