医学分子生物学杂志2025，Vol.22Issue(6)：563-570,8.DOI:10.3870/j.issn.1672-8009.2025.06.005
丁酸钠影响脂肪组织浸润Treg和Th17的比例并改善2型糖尿病小鼠胰岛素敏感性
Effect of Sodium Butyrate on Infiltrated Treg and Th17 Proportion in Adipose Tissues and Insulin Sensitivity in Type 2 Diabetic Mice
摘要
Abstract
Objective To explore the immunological mechanisms of sodium butyrate on impro-ving Type 2 diabetic mouse model's insulin sensitivity.Methods Type 2 diabetic mouse model was established by feeding with a high-fat/high-sucrose diet combined with streptozotocin induc-tion.Thirty male C57BL/6 mice were divided into three groups:control group,model group,mod-el+sodium butyrate group,10 mice in each group.The values of glucose tolerance test and insulin tolerance test were measured.The proportion of infiltrated regulatory T cells(Treg)(％)and T helper type 17(Th17)(％)in mice peripheral blood and adipose tissues were measured by flow cytometry.Western blotting was used to determine the relative expression levels of p-mTOR,mTOR,p-p70S6K and p70S6K in infiltrated Treg and Th17 cells in adipose tissues.Results Glucose toler-ance test results have shown that compared with control group,in model+sodium butyrate group blood glucose levels increased at 20 min(P＜0.05);there was no statistically significant differ-ence in blood glucose at subsequent time points(P＞0.05).Insulin tolerance test results have shown that there was no significant difference in blood glucose levels in model+sodium butyrate group at any time points(P＞0.05).Compared with those in the control group,the percentage(％)of infiltrating Th17 cells in peripheral blood and adipose tissues was increased in the model group(P＜0.05),and the percentage(％)of infiltrating Treg cells was decreased(P＜0.05),and the relative expression levels of p-mTOR and p-p70S6K in infiltrated Treg cells and Th17 cells in adipose tissues were increased(P＜0.05).Compared with the model group,the model+sodium butyrate group significantly reversed the above indicator values(P＜0.05).Conclusion Sodium butyrate treatment could inhibit the activation levels of mTOR/p70S6K signaling pathway,regulate the numbers of Treg cells and Th17 cells in peripheral blood and adipose tissues,and improve insu-lin sensitivity in type 2 diabetes mice model.关键词
2型糖尿病/胰岛素敏感性/丁酸钠/调节性T细胞/辅助T细胞17Key words
type 2 diabetes mellitus/insulin sensitivity/sodium butyrate/regulatory T cells/T helper type 17 cells分类
医药卫生引用本文复制引用
王思瑶,高静,张春雪,罗荔,靳瑾..丁酸钠影响脂肪组织浸润Treg和Th17的比例并改善2型糖尿病小鼠胰岛素敏感性[J].医学分子生物学杂志,2025,22(6):563-570,8.基金项目
新疆医科大学第五附属医院青年科研启航项目(No.XYDWFY-ZR-202308),"天山英才"医药卫生高层次人才培养计划项目(No.TSYC202301B064) This work was supported by grants from Youth Research Launch Project of the Fifth Affiliated Hospital of Xinjiang Medical University(No.XYDWFY-ZR-202308),the"Tianshan Talents"Medical and Health High Level Talent Training Program Project(No.TSYC202301B064) （No.XYDWFY-ZR-202308）
