医学分子生物学杂志2025，Vol.22Issue(6)：579-585,7.DOI:10.3870/j.issn.1672-8009.2025.06.007
circVAPA/miR-377-3p/ZEB2轴对乳腺癌紫杉醇耐药的调控作用
Effect of circRNA VAPA/miR-377-3p/ZEB2 Axis on Paclitaxel Re-sistance of Breast Cancer
摘要
Abstract
Objective To investigate the role of circular RNA vesicle-associated membrane protein-associated protein A(circRNA VAPA)on Zinc-finger E-box binding homeobox 2(ZEB2)in paclitaxel resistance of breast cancer by targeting miR-377-3p.Methods Human breast cancer cell line MCF7 and paclitaxel-resistant strain MCF7/Taxol were cultured,and the expression level of circVAPA,miR-377-3p and ZEB2 were detected and compared.MCF7/Taxol cells were divided into 9 groups.Dual luciferase reporter gene assay was used to detect the targeting relationship be-tween circVAPA and miR-377-3p,and that between miR-377-3p and ZEB2.Cell proliferation inhi-bition rate was measured by CCK-8 assay and the sensitivity to paclitaxel was assessed.Results The expression levels of circVAPA and ZEB2 in the MCF7/Taxol cells were higher than those in the MCF7 cells,and the expression levels of miR-377-3p were lower than those in the MCF7 cells(P＜0.05).The activities of circVAPA and ZEB2 wild-type reporter genes in the miR-377-3p mimic group were both lower than those in the NC group(P＜0.05),while there was no significant difference in the activities of circVAPA and ZEB2 mutant reporter genes(P＞0.05).Compared with those in the NC group,the paclitaxel sensitivity of the si-circVAPA group and the miR-377-3p mim-ic group were increased(P＜0.05),and the expression level of ZEB2 were decreased(P＜0.05).Compared with those in the control plasmid group,the paclitaxel sensitivity in the circVA-PA group was decreased,and the expression level of ZEB2 was increased.Compared with those in the circVAPA group,the paclitaxel sensitivity in the circVAPA+miR-377-3p group was increased,and the expression level of ZEB2 was decreased(P＜0.05).The paclitaxel sensitivity of MCF7/Taxol cells in the si-circVAPA+miR-377-3p inhibitor group was lower than that in the si-circVAPA group(P＜0.05),and the expression level of ZEB2 was higher than that in the si-circVAPA group(P＜0.05).Conclusion The regulation of ZEB2 by circular RNA VAPA/miR-377-3p is related to paclitaxel resistance in breast cancer cells.关键词
乳腺癌/紫杉醇耐药/环状RNA囊泡相关膜蛋白相关蛋白A/miR-377-3p/锌指E盒结合同源框2Key words
breast cancer/paclitaxel resistance/circular RNA vesicle-associated membrane protein-associated protein A/miR-377-3p/Zinc-finger E-box binding homeobox 2分类
医药卫生引用本文复制引用
宋云杰,梁家,徐斌,张朋辉,卫利民..circVAPA/miR-377-3p/ZEB2轴对乳腺癌紫杉醇耐药的调控作用[J].医学分子生物学杂志,2025,22(6):579-585,7.基金项目
河南省医学科技攻关计划联合共建项目(No.LHGJ20200576) This work was supported by a grant from Joint Construction Project of Henan Province Medical Science and Technology Research Program(No.LHGJ20200576) （No.LHGJ20200576）
