医学分子生物学杂志2025，Vol.22Issue(6)：594-600,7.DOI:10.3870/j.issn.1672-8009.2025.06.009
肾癌细胞外泌体上调PD-1抑制CD8+T细胞肿瘤杀伤功能
Renal Cancer Cell Exosomes Inhibit Tumor Killing Function of CD8+T Cells by Upregulation of PD-1
摘要
Abstract
Objective To investigate the effect of renal cancer cell exosome on CD8+T cell tumor killing fuction.Methods HK-2 exo,786-O exo,SN12-PM6 exo,and an equal volume of PBS were co-cultured with CD8+T cells,cytotoxicity assay was used to detect the killing function of CD8+T cells.ELISA was used to detect the secretion of IFN-γ,IL-2 and TNF-α.Western blotting was used to detect the expression level of PD-1 in CD8+T cells.CD8+T cell transfected PD-1 siRNA(si-PD-1)and siRNA Negative Control(si-NC)were co-incubated with 786-O exo and SN12-PM6 exo for 24 hours,respectively.Cytotoxicity experiments were conducted to detect the cytotoxici-ty of CD8+T cells against 786-O and SN12-PM6 cells after transfection.Results Compared with those in the PBS group,the secretion of IFN-γ,IL-2 and TNF-α in the 786-O exo group and SN12-PM6 exo group were significantly downregulated(P＜0.05).The ability to kill 786-O and SN12-PM6 was significantly reduced(P＜0.05),and PD-1 protein expression level was signifi-cantly upregulated(P＜0.05).After co-incubation with 786-O exo and SN12-PM6 exo,the the se-cretion of IFN-γ,IL-2 and TNF-α,as well as the ability to kill 786-O and SN12-PM6 were signifi-cantly increased when compared with those in the CD8+T cells transfected with si-NC(P＜0.05).Conclusion Renal cancer exosome can induce the expression of PD-1 of CD8+T cells to inhibit the tumor killing function.关键词
肾癌/外泌体/CD8+T细胞/程序性死亡受体1Key words
renal cancer/exosome/CD8+T cells/programmed death-1分类
医药卫生引用本文复制引用
王磊,李乾,刘鑫,刘国路,王以金,张成波..肾癌细胞外泌体上调PD-1抑制CD8+T细胞肿瘤杀伤功能[J].医学分子生物学杂志,2025,22(6):594-600,7.基金项目
淮安市科技项目(No.HAB202117) This work was supported by a grant from the Huai'an Science and Technology Project(No.HAB202117) （No.HAB202117）
