医学分子生物学杂志2025，Vol.22Issue(6)：601-606,6.DOI:10.3870/j.issn.1672-8009.2025.06.010
lncRNA MIR100HG促进三阴乳腺癌MDA-MB-231细胞的上皮间质转化
LncRNA MIR100HG Promotes Epithelial-mesenchymal Transition in Triple-negative Breast Cancer MDA-MB-231 Cells
摘要
Abstract
Objective To investigate the effect of long non-coding RNA(lncRNA)MIR100HG on epithelial-mesenchymal transition(EMT)of triple-negative breast cancer MDA-MB-231 cells.Methods MIR100HG was overexpressed or inhibited in the MDA-MB-231 cells,cell proliferation was measured by MTS assay,cell apoptosis rate was analyzed by flow cytometry,and cell migration and invasion were determined by Transwell assay.Western blotting was used to detect the regulatory effect of MIR100HG on epithelial-mesenchymal transition related markers.Results Overexpression of MIR100HG promoted the proliferation,migration and invasion of MDA-MB-231 cells,reduced cell apoptosis,downregulated the expression of epithelial markers and upregulated the expression of mesenchymal markers;Inhibition of MIR100HG produced opposite re-sults.Conclusion Overexpression of LncRNA MIR100HG can promote the proliferation,migration and invasion of MDA-MB-231 cells,reduce the apoptosis of MDA-MB-231 cells,and induce the epithelial-mesenchymal transition of MDA-MB-231 cells.关键词
长链非编码RNA/MIR100HG/上皮-间质转化/MDA-MB-231Key words
long non-coding RNA/MIR100HG/epithelial-mesenchymal transition/MDA-MB-231分类
医药卫生引用本文复制引用
李长春,严文跃,刘根祥,周广军..lncRNA MIR100HG促进三阴乳腺癌MDA-MB-231细胞的上皮间质转化[J].医学分子生物学杂志,2025,22(6):601-606,6.基金项目
盐城市卫健委科技计划项目(No.YK2019003) This work was supported by a grant from the Yancheng Municipal Health Commission Science and Technology Plan Project(No.YK2019003) （No.YK2019003）
