广西植物2025，Vol.45Issue(11)：2042-2055,14.DOI:10.11931/guihaia.gxzw202408003
不同繁殖方式对宿根蔗根系内生微生物群落结构与功能的影响
Effects of different propagation methods on the structure and function of endophytic microbial community in ratoon cane roots
摘要
Abstract
To investigate the effects of different propagation methods on the structures and functions of endophytic bacterial and fungi in ratoon cane roots,the ratoon cane roots were analyzed based on high-throughput sequencing technology.In this study,ratoon cane roots via tissue culture and conventional propagation methods were used as the materials.Meanwhile,the functions of bacterial and fungal communities were also predicted using PICRUSt and FUNGuild softwares,respectively.The results were as follows:(1)The dominant endophytic microbial communities in ratoon cane roots were different between conventional propagation and tissue culture propagation methods.(2)The unique dominant endophytic bacterial genera in ratoon cane roots under the conventional propagation method included Leifsonia,Phyllobacterium,norank_f__Micropepsaceae,Haliangium,Granulicella,Acidothermus,norank_f__Acetobacteraceae and norank_f__Magnetospirillaceae;meanwhile,the unique dominant endophytic fungal genera were Trechispora,Hypochnicium,unclassified_o__Trechisporales,Mycena,Chaetosphaeria,Cladophialophora and Trichoderma.(3)In the ratoon cane roots under the tissue culture propagation method,the unique dominant endophytic bacterial genera included Ralstonia,Amycolatopsis,unclassified_f__Pseudonocardiaceae,Devosia,Streptomyces,Lechevalieria and Kibdelosporangium,and the unique dominant endophytic fungal genera included unclassified_f__Marasmiaceae,unclassified_k__Fungi,Penicillium,Sarocladium,unclassified_c__Agaricomycetes and Setophoma.Compared with the conventional propagation method,the endophytic microbial compositions in ratoon cane roots could be altered by the tissue culture propagation method.(4)The numbers of unique operational taxonomic units(OTUs)of endophytic bacteria and fungi in ratoon cane roots under the tissue culture propagation method was greater than that under the conventional propagation method,indicating higher endophytic microbial diversity and abundance.(5)The functional prediction results showed that the functions of endophytic bacteria in ratoon cane roots were similar between conventional and tissue culture propagation methods,however,the endophyte-plant pathogen-undefined saprotroph group of endophytic fungi was significantly enriched under tissue culture propagation method.In conclusions,endophytic microbial community structures and functions in ratoon cane roots are affected by different propagation methods.All above results also provide a theoretical basis for the subsequent screening,development,and utilization of beneficial functional microbial populations.关键词
甘蔗/宿根蔗/组织培养/常规繁殖/内生微生物Key words
sugarcane/ratoon cane/tissue culture/conventional propagation/endophytic microorganisms分类
农业科技引用本文复制引用
何紫薇,卢文,冯君茜,韦勋,谭宏伟,高轶静,杨尚东,潘永鹏,罗亚伟..不同繁殖方式对宿根蔗根系内生微生物群落结构与功能的影响[J].广西植物,2025,45(11):2042-2055,14.基金项目
国家糖料产业技术体系甘蔗宿根栽培岗位科学项目(CARS-17) （CARS-17）
联合国粮食及农业组织项目(FAO/CPR/3804) （FAO/CPR/3804）
广西科技重大专项(桂科AA22117006). （桂科AA22117006）
