广西植物2025，Vol.45Issue(11)：2056-2066,11.DOI:10.11931/guihaia.gxzw202404040
'长林'油茶良种花粉形态和花粉萌发特性研究
Research on pollen morphology and germination characteristics of Camellia oleifera'Changlin'
摘要
Abstract
To observe the pollen morphological characteristics and explore the influence of nutritional elements on the germination of C.oleifera'Changlin'pollen through in vitro culture.In this experiment,three nationally approved Camellia oleifera'Changlin'series of Camellia,planted in East Guizhou Province,were used as materials.A single-factor and orthogonal tests were conducted using the agar medium germination method.This study was to research the effects of different concentrations of KH2 PO4,H3 BO3,MgCl2,and CO(NH2)2 on in vitro germination of C.oleifera pollen,screen the optimum nutrient ratio for promoting pollen germination and observe the pollen morphology by scanning electron microscope(SEM).The results were as follows:(1)The pollen morphology of C.oleifera'Changlin'was subprolate or spheroidal,the polar view was trilobate-circular,the equatorial view was subprolate or nearly circular,the pollen had three germination furrows,and the outer wall pattern was cerebroid.(2)The single-factor test showed that 0.10-0.15 g·L-1 KH2PO4 had the best effect on pollen germination.The optimal concentrations of the other three elements were 0.05-0.10 g·L-1 for H3 BO3,0.05-0.08 g·L-1 for MgCl2,and 0.15 g·L-1 for CO(NH2)2.(3)The orthogonal test results showed that the treatment effect of 0.10 g·L-1 KH2PO4+0.15 g·L-1 H3BO3+0.08 g·L-1 MgCl2+0.15 g·L-1CO(NH2)2 was the best,which was the best ratio for promoting pollen germination of C.oleifera.The results can provide a reference for assisted pollination of C.oleifera by drone,promoting pollen germination and nutrient solution formulation,and germplasm identification of C.oleifera.关键词
油茶/花粉形态/离体萌发/培养基/花粉营养液Key words
Camellia oleifera/pollen morphology/in vitro germination/medium/pollen nutrient solution分类
农业科技引用本文复制引用
万先琴,高超,宋启玲,孙冬婵,聂艳美,王启梅..'长林'油茶良种花粉形态和花粉萌发特性研究[J].广西植物,2025,45(11):2056-2066,11.基金项目
国家自然科学基金(32360404,32060331) （32360404,32060331）
贵州省科技计划项目(黔科合支撑[2022]重点017号) （黔科合支撑[2022]重点017号）
黔东南州科技计划项目(黔东南科合支撑[2023]03号). （黔东南科合支撑[2023]03号）
