河北医学2025，Vol.31Issue(11)：1761-1766,6.DOI:10.3969/j.issn.1006-6233.2025.11.01
基于AMPK-eNOS通路探讨红景天苷对妊娠高血压大鼠内皮功能障碍的影响
Exploring the Effects of Salidroside on Endothelial Dysfunction in Rats with Hypertensive Disorder of Pregnancy Based on AMPK-eNOS Pathway
摘要
Abstract
Objective:To explore the effects of salidroside(Sal)on endothelial dysfunction in hyperten-sive disorder of pregnancy(HDP)rats based on AMPK-eNOS pathway.Methods:Rat models of HDP were constructed.The successfully modeled rats were randomly classified into HDP group,Sal-L group,Sal-H group,and Sal-H+Compound C(AMPK inhibitor)group,with 12 rats in each group.Another 12 normal healthy rats were made the control group.Blood pressure and 24h urinary protein were measured before and after administration.ELISA was performed to measure serum endothelial function biomarkers and inflammatory factors.HE staining was performed to observe pathological changes in kidney tissue and placenta.Immunohis-tochemistry was used to detect sFLT-1 and PLGF.Western blot was used to measure the AMPK-eNOS path-way related proteins.Results:For the control group,the HDP group showed aggravated renal tissue pathologi-cal damage,increased glomerular edema,and even sclerosis,dilation of renal cysts,thickening of basement membrane,shrinkage of placental structure,reduction of placental villi,and even fibrin like necrosis,and there was an increase in vacuolated cells in the trophoblast layer and infiltration of inflammatory cells.The mean arterial pressure,24h UTP,ET-1,TXB2,TNF-α,IL-6,IL-17 levels,and sFLT-1 expression in-creased,while the NO level and PLGF,p-AMPK/AMPK,p-AKT/AKT,and p-eNOS/eNOS expression de-creased(P<0.05).The pathological damage of renal tissue and placental tissue in the Sal-L group and Sal-H group was alleviated compared with the HDP group,with a decrease of the mean arterial pressure,24h UTP,ET-1,TXB2,TNF-α,IL-6,IL-17 levels,and sFLT-1,and an increase in the NO level and PL-GF,p-AMPK/AMPK,p-AKT/AKT,and p-eNOS/eNOS expression(P<0.05).The Sal-H+Compound C group showed an increase in mean arterial pressure,24h UTP,ET-1,TXB2,TNF-α,IL-6,IL-17 levels,and sFLT-1 expression,and a decrease in NO level and PLGF,p-AMPK/AMPK,p-AKT/AKT,and p-eNOS/eNOS expression than Sal-H group(P<0.05).Conclusion:Salidroside can improve endothelial dys-function in HDP rats and is associated with the activation of AMPK-eNOS pathway.关键词
AMPK-eNOS通路/红景天苷/妊娠高血压/内皮功能障碍Key words
AMPK-eNOS pathway/Salidroside/Hypertensive disorder of pregnancy/Endothe-lial dysfunction引用本文复制引用
郑婷华,宋慧珍,王丽丽,宋志慧..基于AMPK-eNOS通路探讨红景天苷对妊娠高血压大鼠内皮功能障碍的影响[J].河北医学,2025,31(11):1761-1766,6.基金项目
河北省医学科学研究重点课题计划项目,(编号:20231751) （编号:20231751）
