河北医学2025，Vol.31Issue(11)：1773-1780,8.DOI:10.3969/j.issn.1006-6233.2025.11.03
基于NGF/Akt/GSK3β通路探讨胫骨横向骨搬移对糖尿病足大鼠创面修复的机制研究
Mechanism of Tibial Transverse Bone Transfer on Trauma Repair in Diabetic Foot Rats Based on the NGF/Akt/GSK3β Pathway
摘要
Abstract
Objective:To investigate the effect of tibial transverse bone relocation on trauma repair in diabetic foot rats,and to detect the expression of factors related to the NGF/Akt/GSK3β pathway to elucidate its possible mechanism of action.Methods:Forty SPF grade rats were selected,of which 10 were in the con-trol group and the remaining 30 were modeled as diabetic foot rats.28 rats finally completed the modeling and were divided into model group(10 rats),tibial transverse bone transfer group(9 rats),and tibial transverse bone transfer+MK-2206 group(9 rats).Wound healing rate,serum biochemical indexes,fasting blood glu-cose,oxidative stress indexes,capillary number,proportion of EPCs,VEGF,inflammatory factors and NGF/Akt/GSK3β pathway-related protein expression were compared between the groups.Results:Compared with the control group,TNF-α,fasting glucose,CRP,LDL-C,MDA,TG,IL-6,TC,and GSK3β protein ex-pression were elevated,and the levels of Akt protein expression,capillary count,NGF protein expression,SOD,EPCs ratio,HDL-C,VEGF,and trauma healing rate in the model and tibia transverse bone relocation+MK-2206 group decreased,(P<0.05);Compared with the model group,the ratio of EPCs,SOD,NGF protein expression,capillary number,HDL-C,Akt protein expression,VEGF,and wound healing rate levels were increased,and the levels of TNF-α,MDA,LDL-C,fasting glucose,GSK3β protein expression,TC,CRP,TG,and IL-6 were decreased in the tibia transverse bone relocation group(P<0.05);Compared with the tibial transverse bone transfer group,the TNF-α,fasting glucose,IL-6,TC,MDA,CRP,TG,GSK3β protein expression,and LDL-C levels were increased in the tibial transverse bone transfer+MK-2206 group,and the levels of VEGF,SOD,Akt protein expression,HDL-C,NGF protein expression,the proportion of EPCs,the traumatic wound healing rate,and the level of the capillary number were decreased(P<0.05).Conclusion:Transverse tibial bone relocation promotes trauma repair and improves the expression of serum bi-ochemical indexes,oxidative stress,capillary count,and inflammatory factors in diabetic foot rats,and its mechanism may be related to the NGF/Akt/GSK3β pathway.关键词
糖尿病足/神经生长因子/蛋白激酶B/糖原合成酶激酶3β/胫骨横向骨搬移/创面修复Key words
Diabetic foot/Nerve growth factor/protein kinase B/glycogen synthase kinase 3β/Transverse tibial bone displacement/Wound repair引用本文复制引用
徐凯,刘其赞,贺达,陈广先,郑宁宁,夏洪乐..基于NGF/Akt/GSK3β通路探讨胫骨横向骨搬移对糖尿病足大鼠创面修复的机制研究[J].河北医学,2025,31(11):1773-1780,8.基金项目
河北省中医药管理局科研计划项目,(编号:2025554) （编号:2025554）
