河北医学2025，Vol.31Issue(11)：1780-1786,7.DOI:10.3969/j.issn.1006-6233.2025.11.04
艾司氯胺酮对阻塞性睡眠呼吸暂停综合征大鼠认知功能与Shh/Ptch1通路的影响
The Effect of Esketamine on Cognitive Function and Shh/Ptch1 Pathway in Rats with Obstructive Sleep Apnea Syndrome
摘要
Abstract
Objective:To explore the effects of Esketamine(ESK)on hippocampal tissue injury and cognitive function in rats with obstructive sleep apnea syndrome(OSAS),as well as its regulatory role in the sonic hedgehog factor(Shh)/complement homologous 1(Ptch1)pathway.Methods:Totally 72 male SD rats were stochastically separated into a control group,a model group(OSAS group),a low-dose ESK group(ESK-L group),a high-dose ESK group(ESK-H group),a Shh/Ptch1 pathway activator group(Purmor-phoamine group),and a high-dose ESK+Shh/Ptch1 pathway inhibitor group(ESK-H+Cyclopamine group),each with 12 rats.Except for the control group,OSAS models were constructed in all other groups.The cogni-tive ability(Morris water maze test)and blood oxygen saturation(blood oxygen monitor)of rats in each group were tested.The hippocampal tissue of rats was taken to detect the inflammatory factors and oxidative stress factors(ELISA method),pathological changes in hippocampal tissue(HE staining),neuronal apoptosis(TUNEL method),neuronal ultrastructure(transmission electron microscopy method),and Shh/Ptch1 path-way proteins(Western blot method).Results:The OSAS group had prominently lower blood oxygen satura-tion,proportion of plateau dwelling time,IL-10 content,SOD activity,and Shh/Ptch1 pathway proteins in hippocampal tissue,and prominently higher escape latency,IL-6 and MDA contents in hippocampal tissue,and neuronal apoptosis rate(P<0.05).ESK could prominently increase blood oxygen saturation,proportion of plateau dwelling time,IL-10 content,SOD activity,and Shh/Ptch1 pathway proteins in hippocampal tis-sue,and prominently reduce escape latency,IL-6 and MDA contents in hippocampal tissue,and neuronal apoptosis rate.The changes in these indicators showed a dose-response relationship with ESK(P<0.05).Cyclopamine could reverse the improvement effects of ESK on hippocampal tissue damage and cognitive func-tion in OSAS rats(P<0.05).Conclusion:ESK can inhibit inflammation and oxidative stress response in hip-pocampal tissue,alleviate neuronal apoptosis and pathological damage in hippocampal tissue,and improve cognitive ability.Its mechanism of action may be achieved by activating the Shh/Ptch1 pathway.关键词
艾司氯胺酮/阻塞性睡眠呼吸暂停综合征/海马组织/认知功能/Shh/Ptch1通路Key words
Esketamine/Obstructive sleep apnea syndrome/Hippocampal tissue/Cognitive function/Shh/Ptch1 pathway引用本文复制引用
谷昆峰,马文女,董慧咏,赵建辉..艾司氯胺酮对阻塞性睡眠呼吸暂停综合征大鼠认知功能与Shh/Ptch1通路的影响[J].河北医学,2025,31(11):1780-1786,7.基金项目
石家庄市科学技术研究与发展指导计划,(编号:191460443) （编号:191460443）
